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本文引用的文献

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Complete nucleotide sequence of a soybean actin gene.大豆肌动蛋白基因的完整核苷酸序列。
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2
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.还原和S-羧甲基化蛋白质的制备及酶促水解
J Biol Chem. 1963 Feb;238:622-7.
3
The oxidation of ribonuclease with performic acid.用过甲酸氧化核糖核酸酶。
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A gas-liquid solid phase peptide and protein sequenator.一种气-液-固相肽和蛋白质测序仪。
J Biol Chem. 1981 Aug 10;256(15):7990-7.
5
Complete nucleotide sequence of a sea urchin actin gene.海胆肌动蛋白基因的完整核苷酸序列。
Nucleic Acids Res. 1982 Jul 10;10(13):4081-92. doi: 10.1093/nar/10.13.4081.
6
Vegetative Dictyostelium cells containing 17 actin genes express a single major actin.含有17个肌动蛋白基因的盘基网柄菌营养细胞表达一种主要的肌动蛋白。
Nature. 1980 Apr 3;284(5755):475-7. doi: 10.1038/284475a0.
7
Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography.采用超灵敏梯度高效液相色谱法分析苯乙内酰硫脲。
Methods Enzymol. 1983;91:486-93. doi: 10.1016/s0076-6879(83)91045-5.
8
Genes encoding actin in higher plants: intron positions are highly conserved but the coding sequences are not.高等植物中编码肌动蛋白的基因：内含子位置高度保守，但编码序列并非如此。
J Mol Appl Genet. 1983;2(1):111-26.
9
Phalloidin enhances actin assembly by preventing monomer dissociation.鬼笔环肽通过阻止单体解离来增强肌动蛋白组装。
J Cell Biol. 1984 Aug;99(2):529-35. doi: 10.1083/jcb.99.2.529.
10
Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence.两个人类β-肌动蛋白相关加工基因的结构，其中一个位于一个简单重复序列旁边。
EMBO J. 1983;2(5):757-61. doi: 10.1002/j.1460-2075.1983.tb01496.x.

F-肌动蛋白的鬼笔环肽结合位点。

The phalloidin binding site of F-actin.

作者信息

Vandekerckhove J, Deboben A, Nassal M, Wieland T

出版信息

EMBO J. 1985 Nov;4(11):2815-8. doi: 10.1002/j.1460-2075.1985.tb04008.x.

DOI:10.1002/j.1460-2075.1985.tb04008.x
PMID:4065095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554583/
Abstract

Tritium-containing affinity-labelling derivatives of phalloidin, an alkylating iodoacetyl compound (EAL) and a photolabile, carbene generating diazirine (PAL), have been reacted with rabbit muscle actin, the former after protection of thiol groups with N-ethylmaleimide. Labelled peptides generated by tryptic and/or thermolysin digestion were isolated by paper peptide mapping and characterized by determination of their amino acid sequences. EAL binds to methionine-119 and methionine-355; PAL binds to glutamic acid-117. These residues are located in regions with extremely conserved amino acid sequences. The cleft between the two domains of the actin monomer is suggested as the possible binding site for phalloidin.

摘要

用含有氚的鬼笔环肽亲和标记衍生物、一种烷基化碘乙酰化合物（EAL）和一种光不稳定的、能产生卡宾的重氮丙啶（PAL）与兔肌肉肌动蛋白反应，前者在用N - 乙基马来酰亚胺保护巯基后进行反应。通过纸层析肽图谱分离经胰蛋白酶和/或嗜热菌蛋白酶消化产生的标记肽，并通过测定其氨基酸序列进行表征。EAL与甲硫氨酸 - 119和甲硫氨酸 - 355结合；PAL与谷氨酸 - 117结合。这些残基位于氨基酸序列极其保守的区域。肌动蛋白单体两个结构域之间的裂隙被认为是鬼笔环肽可能的结合位点。