Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
Int J Mol Sci. 2020 Mar 6;21(5):1828. doi: 10.3390/ijms21051828.
Jasmonates (JAs), the group of lipid-derived hormones, were found to control the defense responses in a myriad of plants. Meaningfully, the macrolactones of 12-hydroxy jasmonate isoleucine (12OH-JA-Ile) were reported to induce the defensive response of wild tobacco. However, little to nothing has been known about the elicitation effect of JA-Ile-macrolactones on woody plants to harmful organisms, let alone its underlying mechanisms. Here, we first optimized the synthetic routine using mild toxic reagent isobutyl chloroformate instead of ethyl chloroformate for conjugation, and we used acetonitrile (MeCN) instead of ethyl alcohol for the better dissolution of -toluenesulfonic acid (-TsOH) to gain JA-Ile-macrolactones. JA-Ile-macrolactone -treated tea plants significantly inhibited the larvae weight gain of larvae and the lesions caused by . Furthermore, the expression level of was significantly upregulated in -treated leaves. Meanwhile, reduced the accumulation of eriodictyol 7-O-glucuronide (EDG) in tea plants, which was confirmed to promote the growth rate of larvae by artificial diet assay. In conclusion, our study proved that the exogenous application of could induce the tea plant resistance both to herbivore and pathogen , and EDG was identified as one of the secondary metabolites that could influence the growth rate of , but it did not directly influence the infection of . Further research should be carried out to clarify the mechanism through which induces tea plant resistance to .
茉莉酸（JAs）是一类脂质衍生的激素，被发现可以控制多种植物的防御反应。有意思的是，12-羟基茉莉酸异亮氨酸（12OH-JA-Ile）的大环内酯被报道可以诱导野生烟草的防御反应。然而，对于 JA-Ile-大环内酯对有害生物的木本植物的诱抗作用，以及其潜在的机制，人们知之甚少。在这里，我们首次使用温和毒性试剂异丁基氯甲酸酯代替乙基氯甲酸酯进行共轭，优化了合成常规，并用乙腈（MeCN）代替乙醇以更好地溶解对甲苯磺酸（-TsOH），从而获得 JA-Ile-大环内酯。JA-Ile-大环内酯处理的茶树显著抑制了 幼虫的体重增加和 引起的损伤。此外，在 -处理的叶片中， 的表达水平显著上调。同时， 减少了茶树中埃里多蒂醇 7-O-葡萄糖醛酸苷（EDG）的积累，通过人工饲料试验证实 EDG 可以促进 幼虫的生长速度。总之，我们的研究证明了外源施用 JA-Ile 可以诱导茶树对草食性 和病原菌 的抗性，并且 EDG 被鉴定为可以影响 幼虫生长速度的次生代谢物之一，但它并不直接影响 的感染。应该进一步研究阐明 JA-Ile 诱导茶树对 的抗性的机制。
