应用纳米二次离子质谱仪（NanoSIMS）和同位素标记分析植物铁螯合剂烟酰胺的空间分布。

Application of NanoSIMS and isotopic labeling to analyze the spatial distribution of the plant iron chelator nicotianamine.

作者信息

Nozoye Tomoko, Takeuchi Miyuki, Hollmann Fabian, Clemens Stephan

机构信息

Meiji Gakuin University, Center for Liberal Arts, Yokohama, Japan; The University of Tokyo, Department of Global Agricultural Sciences, Laboratory of Plant Biotechnology, Tokyo, Japan.

The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Biomaterial Sciences, Tokyo, Japan.

出版信息

J Biol Chem. 2025 Jul 11;301(8):110478. doi: 10.1016/j.jbc.2025.110478.

DOI:10.1016/j.jbc.2025.110478

PMID:40653201

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12344255/

Abstract

Nicotianamine (NA) is an iron (Fe) chelator responsible for the Fe translocation and transport within the plant body and cells. NA is also hypothesized to be involved in Fe homeostasis as a Fe deficiency signal transducer in plants. This study visualized the distribution of stable isotope-labeled NA in plants by high-resolution secondary ion mass spectrometry (NanoSIMS) to better understand the mechanisms of NA-mediated Fe translocation, transport, and Fe homeostasis. Stable isotope-labeled NA (N-NA) was produced by heterologous expression of an NA synthase in yeast and supplied to wild type (WT) tomato plants and the NA-free mutant chloronerva (chln). NanoSIMS showed that N-NA signals were concentrated in tissue surrounding xylem vessels, where Fe accumulated in both WT and chln. Our results show that NanoSIMS is a powerful tool for investigating NA localization directly in plants.

摘要

烟酰胺（NA）是一种铁（Fe）螯合剂，负责植物体内和细胞内铁的转运。NA也被认为作为植物中铁缺乏信号转导器参与铁稳态调节。本研究通过高分辨率二次离子质谱（NanoSIMS）观察稳定同位素标记的NA在植物中的分布，以更好地理解NA介导的铁转运、运输及铁稳态调节机制。稳定同位素标记的NA（N-NA）通过在酵母中异源表达NA合酶产生，并提供给野生型（WT）番茄植株和无NA突变体chloronerva（chln）。NanoSIMS显示N-NA信号集中在木质部导管周围的组织中，WT和chln中的铁都在该部位积累。我们的结果表明，NanoSIMS是直接研究植物中NA定位的有力工具。