Nakagawa Keisuke, Miyawaki Shingo, Moribe Junji, Masatani Tatsunori, Saito Taizo, Takashima Yasuhiro
Faculty of Applied Biological Science, Joint Department of Veterinary Medicine, Gifu University, Gifu, Japan.
Joint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
J Vet Med Sci. 2025 Sep 5;87(9):1107-1111. doi: 10.1292/jvms.24-0406. Epub 2025 Jul 11.
Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.
尽管大多数冠状病毒被认为起源于蝙蝠，但关于蝙蝠是否仍对当前流行的冠状病毒易感的信息却很少。我们通过对细胞进行连续传代，从伏翼（Pipistrellus abramus，简称P. abramus）的肾脏中生成了一个连续细胞系，该细胞系可在无任何永生化过程的情况下增殖超过80代。我们发现，在胰蛋白酶存在的情况下，猪流行性腹泻病毒（PEDV）和牛冠状病毒（BCoV）能够感染P. abramus来源的细胞并产生子代病毒，尽管复制效率相当低。这些发现表明，伏翼可能对PEDV和BCoV易感，并可能作为这些病毒的传播者发挥潜在作用。
