Chen Yinping, Cao Bo, Lu Yuzhi, Zheng Yan, Sun Yong, Li Qian, Yuan Qiaoling, Zhang Xiaolan
School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, China.
Key Laboratory of Strategic Mineral Resources in the Upper Yellow River, Ministry of Natural Resources, Lanzhou, China.
Sci Rep. 2025 Jul 13;15(1):25310. doi: 10.1038/s41598-025-11538-1.
Biodegradable chelating agents and nitrogen (N) fertilizers are widely used for the remediation of heavy metal-contaminated soils due to their ability to promote plant growth, enhance metal mobility and facilitate plant uptake. In this study, a pot experiment was conducted to investigate the effects of nitrate nitrogen (N-NO), ammonium nitrogen (N-NH) and amide nitrogen (N-amide) with different concentrations of 25, 50 and 75 mg N·kg combined with (S,S)-ethylenediaminedisuccinic acid (EDDS) (1 mmol·L) on growth, biomass and phytoextraction efficiency of Bidens. pilosa L. and the bioavailability of cadmiun (Cd) in 15 mg·kg Cd-contaminated soil. The results demonstrated that the application of 50 mg N·kg N-NO and EDDS not only promoted plant growth and increased biomass, it also changed Cd distribution between operational fractions, increased available Cd content in soil, thereby maximizing the Cd uptake, thus enhancing the Cd enrichment capability of B. pilosa L. This combination achieved the maximum phytoextraction efficiency of 12.12% and the maximum soil Cd removal of 12.38% across all treatments. The comprehensive score obtained using the membership function method also showed that the treatment with 50 mg N·kg N-NO and EDDS had the highest score. In conclusion, combination of 50 mg N·kg N-NO and EDDS resulted in the greatest biomass production, and highest phytoremediation efficiency, indicating that it has great potential for application in phytoremediation with B. pilosa L. in Cd-contaminated soil.
可生物降解螯合剂和氮肥因其促进植物生长、提高金属迁移率和促进植物吸收的能力，被广泛用于修复重金属污染土壤。本研究通过盆栽试验，研究了不同浓度（25、50和75 mg N·kg）的硝态氮（N-NO）、铵态氮（N-NH）和酰胺态氮（N-酰胺）与（S,S）-乙二胺二琥珀酸（EDDS）（1 mmol·L）组合对三叶鬼针草生长、生物量和植物提取效率以及15 mg·kg镉污染土壤中镉生物有效性的影响。结果表明，施用50 mg N·kg N-NO和EDDS不仅促进了植物生长、增加了生物量，还改变了镉在不同操作组分之间的分布，增加了土壤中有效镉含量，从而使镉吸收最大化，进而提高了三叶鬼针草的镉富集能力。在所有处理中，该组合实现了最高的植物提取效率12.12%和最大的土壤镉去除率12.38%。利用隶属函数法得到的综合得分也表明，50 mg N·kg N-NO和EDDS处理得分最高。总之，50 mg N·kg N-NO和EDDS的组合产生了最大的生物量产量和最高的植物修复效率，表明其在镉污染土壤中三叶鬼针草植物修复方面具有很大的应用潜力。
