PereiraTavares Antonio J, Aranda-Michel Liam, Hahn Scott, Coffin Brian D, Ashraf Syed Faaz, Szafron Jason M, Straub Adam C, Shiwarski Daniel J
Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Vascular Medicine Institute, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
bioRxiv. 2025 May 4:2025.04.29.651300. doi: 10.1101/2025.04.29.651300.
Pressure myography, the standard for assessing vascular mechanics and vasoreactivity, is costly ($40,000+), has low throughput, and is limited to static fluid flow. Here, we developed HemoLens, an open-source 3D-printed pressure myography system for ~$700. HemoLens features compact micromanipulators, incremental in-line pressure control, physiological temperature regulation, and modular pulse pressure control between normotensive and hypertensive levels. HemoLen's efficacy was demonstrated by delineation of physiological reactivity and pathological mechanical phenotypes using native mouse arteries and bioprinted acellular scaffolds. Wildtype vessels show greater distention (124.3 vs. 43.07 μm) and increased dynamic compliance compared to diseased vessels. Small diameter (450 μm) collagen-based artery-like scaffolds are FRESH bioprinted to mimic hypertensive vascular stiffening. Engineered hypertensive vessels demonstrate increased burst pressure (464 mmHg) and reduced dynamic compliance reminiscent of diseased arteries. Together, HemoLens lowers the barrier to entry in pressure myography research by serving as a comprehensive low-cost system for native and engineered vessel characterization.
压力肌动描记法作为评估血管力学和血管反应性的标准方法，成本高昂（超过4万美元），通量低，并且仅限于静态流体流动。在此，我们开发了HemoLens，这是一种开源的3D打印压力肌动描记系统，成本约为700美元。HemoLens具有紧凑的微操纵器、增量式在线压力控制、生理温度调节以及在正常血压和高血压水平之间的模块化脉压控制。通过使用天然小鼠动脉和生物打印的无细胞支架描绘生理反应性和病理力学表型，证明了HemoLen的有效性。与患病血管相比，野生型血管显示出更大的扩张（124.3对43.07μm）和增加的动态顺应性。小直径（450μm）基于胶原蛋白的动脉样支架通过FRESH生物打印来模拟高血压血管僵硬。工程化的高血压血管表现出增加的破裂压力（464 mmHg）和降低的动态顺应性，类似于患病动脉。总之，HemoLens作为一种用于天然和工程化血管表征的全面低成本系统，降低了压力肌动描记法研究的入门门槛。
