Konefał Anna, Kalisz Justyna, Stelmach Emilia, Piątek Piotr, Maksymiuk Krzysztof, Michalska Agata
Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Lukasiewicz Research Network, Industrial Chemistry Institute, Rydygiera 8, 01-793 Warsaw, Poland.
ACS Omega. 2025 Jun 27;10(26):28429-28435. doi: 10.1021/acsomega.5c03974. eCollection 2025 Jul 8.
This work compared the performance of ion-selective optodes in different nanostructural formats: nanodroplets and nanofibers prepared from biocompatible materials using classical ionophores and ion exchangers. Nanodroplets tested contained minute amounts of plasticizer acetyl tributyl citrate stabilized with poly-(vinyl alcohol) and were suspended in the aqueous phase. It was shown that this system was not directly transferable to the nanofiber format; thus, nanofibers obtained from polycaprolactone were used as the support material. Both nanodroplets and nanofibers were used in Nile blue/Nile red-based systems operating as novel ion-selective sensor optical transducers, allowing sensors to be applied in unbuffered samples. As a model system, calcium sensors were prepared and tested. It was shown that, regardless of format, optodes were characterized by increased emission intensity at the Nile red characteristic wavelength (605 nm) for increasing calcium ion concentration in the sample. Nile blue emission changes for varying analyte concentrations were specific for the format (they changed for nanodroplets, whereas for nanofibers, they were similar within the range of experimental error). For both systems, a linear dependence of the ratio of emission intensity was recorded for Nile red and Nile blue characteristic wavelength on the changes in analyte concentration in the sample within the range from 10 to 10 M. The unique advantages of using nanofiber mats include the determination of optical signals for both sensors present in solution and probes removed from solution; moreover, the same sensor can be transferred between different solutions. This is a clear advantage of the application of nanofiber mats as sensors, especially when used on a dedicated, 3D-made holder.
使用经典离子载体和离子交换剂由生物相容性材料制备的纳米液滴和纳米纤维。测试的纳米液滴含有微量用聚（乙烯醇）稳定的增塑剂乙酰柠檬酸三丁酯，并悬浮在水相中。结果表明，该系统不能直接转换为纳米纤维形式；因此，使用由聚己内酯获得的纳米纤维作为支撑材料。纳米液滴和纳米纤维都用于基于尼罗蓝/尼罗红的系统，作为新型离子选择性传感器的光学换能器，使传感器能够应用于未缓冲的样品中。作为模型系统，制备并测试了钙传感器。结果表明，无论形式如何，对于样品中钙离子浓度的增加，光极在尼罗红特征波长（605nm）处的发射强度都会增加。尼罗蓝发射随分析物浓度变化而变化的情况因形式而异（纳米液滴会发生变化，而对于纳米纤维来说，在实验误差范围内它们是相似的）。对于这两个系统，在10到10M范围内，记录了尼罗红和尼罗蓝特征波长处发射强度比与样品中分析物浓度变化之间的线性关系。使用纳米纤维垫的独特优势包括能够测定溶液中存在的两种传感器以及从溶液中取出的探针的光信号；此外，可以在不同溶液之间转移相同的传感器。这是将纳米纤维垫用作传感器的明显优势，尤其是在专用的3D制作支架上使用时。
