Faculty of Chemistry, University of Warsaw , Pasteura 1, 02-093 Warsaw, Poland.
Anal Chem. 2016 Jun 7;88(11):5644-8. doi: 10.1021/acs.analchem.6b00737. Epub 2016 May 11.
A novel type of ion-selective nano-optode is proposed, in which a conjugated polymer is used as optical transducer and nanoprobe material. Thus, contrary to most of the proposed optodes, the response does not require presence of pH-sensitive dye in the sensor. The conjugated polymer nanosensor material is in partially oxidized form-it is bearing positive charges and its emission is quenched. The receptor is an optically silent uncharged ionophore selective for the analyte cation. When a binding event occurs, positive charges are formed in the nanosphere, leading to a decrease in the oxidation state of the polymer, in the absence of redox potential change, resulting in increased emission. This general approach herein proposed results in a simple sensor, benefitting from a novel optical transduction mechanism and high lipophilicity of the polymer matrix that results in linear responses over a broad concentration range of analyte. For the model system studied, the linear dependence of emission intensity on the logarithm of analyte (K(+)) concentration was obtained for a broad range from 10(-5) M to 0.1 M.
提出了一种新型的离子选择性纳米光极，其中使用共轭聚合物作为光学换能器和纳米探针材料。因此，与大多数提出的光极不同，该响应不需要在传感器中存在 pH 敏感染料。共轭聚合物纳米传感器材料处于部分氧化状态——带有正电荷，其发射被猝灭。受体是一种光学上无声的、对分析物阳离子具有选择性的不带电荷的离子载体。当发生结合事件时，在纳米球中形成正电荷，导致聚合物的氧化态降低，在没有氧化还原电位变化的情况下，导致发射增加。本文提出的这种通用方法导致了一种简单的传感器，得益于新型的光学转换机制和聚合物基质的高亲脂性，这导致了在分析物的宽浓度范围内的线性响应。对于所研究的模型体系，在从 10(-5) M 到 0.1 M 的宽范围内获得了发射强度与分析物（K(+））浓度对数的线性依赖性。
