Zhong Jiehao, Chen Qingxiang, Deng Xiaojie, Guan Yongpeng, He Qing, Nethmini Rajapakshalage Thashikala, Tang Jing, Hou Qinghua, Li Xiaolei, Jiang Gonglingxia, Huang Laizhen, Dong Ke, Li Nan
Key Laboratory of Climate, Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong Province, Department of Oceanography, Key Laboratory for Coastal Ocean Variation and Disaster Prediction, College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang, China.
Department of Biological Sciences, Kyonggi University, Suwon-si, Republic of Korea.
Front Microbiol. 2025 Jun 27;16:1620942. doi: 10.3389/fmicb.2025.1620942. eCollection 2025.
Mycoplankton are essential for biogeochemical cycles in natural water bodies. However, the distribution of the mycoplanktonic community and its community-level change points in subtropical estuaries remain unclear. In this study, we employed 18S rRNA high-throughput sequencing to explore the mycoplanktonic community structure and environmental thresholds in the Dafengjiang River Estuary. Agaricostilbomycetes and Saccharomycetes are the dominant classes in the Dafengjiang River Estuary. The alpha and beta diversities of the mycoplanktonic communities showed significant differences ( < 0.05) across the seasons. Distance-based redundancy analysis (db-RDA) suggested that the main driver of the total community was eutrophication level, and the key factors for oligotrophication, medium eutrophication, and high eutrophication were dissolved inorganic phosphorus (DIP), ammonium (NH ), and chlorophyll- (Chl-), respectively. Threshold Indicator Taxa Analysis (TITAN) exhibited the community-level change points of mycoplankton along the eutrophication gradients were DIP (6-15.5 μg/L), NH (61.5-62.5 μg/L) and Chl- (2.55-9.3 μg/L), respectively. Random forest analysis revealed that , and were sensitive to eutrophication status and could serve as bioindicator genera for environmental changes. Overall, our study enhances our understanding of the diversity and community-level change points of mycoplankton in subtropical estuaries and lays the theoretical foundation for the environmental monitoring of subtropical estuaries.
海洋真菌浮游生物对于天然水体中的生物地球化学循环至关重要。然而，亚热带河口地区海洋真菌浮游生物群落的分布及其群落水平的变化点仍不清楚。在本研究中，我们采用18S rRNA高通量测序技术，探究了大风江河口海洋真菌浮游生物的群落结构和环境阈值。木耳菌纲和酵母纲是大风江河口的优势纲。海洋真菌浮游生物群落的α多样性和β多样性在不同季节间存在显著差异（P<0.05）。基于距离的冗余分析（db-RDA）表明，总群落的主要驱动因素是富营养化水平，贫营养化、中度富营养化和高度富营养化的关键因素分别是溶解无机磷（DIP）、铵（NH₄⁺）和叶绿素-a（Chl-a）。阈值指示分类群分析（TITAN）显示，海洋真菌浮游生物沿富营养化梯度的群落水平变化点分别为DIP（6 - 15.5μg/L）、NH₄⁺（61.5 - 62.5μg/L）和Chl-a（2.55 - 9.3μg/L）。随机森林分析表明，曲霉属、青霉属和念珠菌属对富营养化状态敏感，可作为环境变化的生物指示属。总体而言，我们的研究增进了我们对亚热带河口海洋真菌浮游生物多样性和群落水平变化点的理解，为亚热带河口的环境监测奠定了理论基础。
