Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Defense Road Campus, Lahore, Pakistan.
Department of Pathology, Akhtar Saeed Medical & Dental College, Lahore, Pakistan.
Appl Biochem Biotechnol. 2024 Mar;196(3):1419-1434. doi: 10.1007/s12010-023-04618-3. Epub 2023 Jul 7.
Lyophilized human amniotic membrane (HAM) and silver nanoparticles (AgNPs) have multispectral applications as a biological dressing. The present study focuses on the safety aspects of HAM coated with colistin and AgNPs (HACoN) dressing in relation to its structural and hematological changes. Four dressing groups were designed for the study, HAM, HAM coated with colistin (HACo), HAM coated with AgNPs (HAN), and HAM coated with colistin (HACo) and HACoN. Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) were utilized for constitutional analysis. Biological safety was checked by applying HAM of all groups on open excisional burn wounds on Sprague-Dawley rats for 21 days. The skin, kidneys, liver, and spleen were removed, and histological analysis was performed for detailed structural analysis. Oxidative stress was assessed using homogenate from newly generated skin. No structural or biochemical change was observed in any of the study groups as observed by SEM and FTIR. After 21 days of grafting, wounds were healed properly with normal skin, and no anomaly was observed in related to kidneys, spleen, and liver. Some of antioxidant enzymes were increased, while malondialdehyde which is a reactive oxygen species was reduced in the skin tissue homogenate of HACoN group. Impregnation of colistin and AgNPs in combination on HAM has no effects on hematological and structural constitution of HAM. It leaves no obvious change in vital organs of rats and improves oxidative stress and inflammation. Hence, it can be claimed that HACoN is a biologically safe antibacterial dressing.
冻干人羊膜（HAM）和纳米银颗粒（AgNPs）作为生物敷料具有多光谱应用。本研究侧重于载有黏菌素和 AgNPs 的 HAM （HACoN）敷料在结构和血液学变化方面的安全性方面。研究设计了 4 个敷料组，即 HAM、载有黏菌素的 HAM（HACo）、载有 AgNPs 的 HAM（HAN）和载有黏菌素和 AgNPs 的 HAM（HACoN）。扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）用于结构分析。通过将所有组的 HAM 应用于 Sprague-Dawley 大鼠的开放性切创烧伤创面 21 天，检查生物安全性。切除皮肤、肾脏、肝脏和脾脏，并进行组织学分析以进行详细的结构分析。使用新生成的皮肤匀浆评估氧化应激。SEM 和 FTIR 观察到，所有研究组均未观察到结构或生化变化。在移植后 21 天，伤口愈合良好，皮肤正常，与肾脏、脾脏和肝脏相关的无异常。一些抗氧化酶增加，而丙二醛（一种活性氧）在 HACoN 组皮肤组织匀浆中减少。将黏菌素和 AgNPs 联合浸渍在 HAM 上对 HAM 的血液学和结构组成没有影响。它不会对大鼠的重要器官造成明显改变，并改善氧化应激和炎症。因此，可以说 HACoN 是一种生物安全的抗菌敷料。
