Merski J A, Meyers M C
Food Chem Toxicol. 1985 Oct;23(10):923-30. doi: 10.1016/0278-6915(85)90109-7.
Cytoplasmic vacuolation of renal proximal tubular epithelial cells was studied in rats following administration of nitrilotriacetate (NTA) or sucrose. Sucrose was administered at both a high dose (29.2 mmol/kg) and low dose (7.3 mmol/kg) by ip injection. Both levels of sucrose induced severe vacuolation of the renal proximal tubular epithelium, as observed by light microscopy. However, at the high dose, the vacuolation was widespread, affecting essentially all the proximal tubules, while at the low dose, the lesion was distributed in a multifocal pattern. Nitrilotriacetate administered by gavage at a level of 7.3 mmol/kg also induced severe cytoplasmic vacuolation in the renal proximal tubular epithelium. The distribution of this lesion was multifocal and indistinguishable from that caused by the 7.3-mmol/kg dose of sucrose. Electron-microscopic examination of vacuolated tubule cells demonstrated that, in both the NTA- and sucrose-treated animals, the lesion was due to changes in the endocytotic/lysosomal system. The nuclei, mitochondria, golgi and endoplasmic reticulum and the highly convoluted areas of the basal membrane appeared normal in both the vacuolated and non-vacuolated tubule cells of rats given either NTA or sucrose.
在给大鼠注射次氮基三乙酸（NTA）或蔗糖后，对其肾近端小管上皮细胞的细胞质空泡化进行了研究。通过腹腔注射给予高剂量（29.2 mmol/kg）和低剂量（7.3 mmol/kg）的蔗糖。光学显微镜观察发现，两种剂量的蔗糖均诱导了肾近端小管上皮的严重空泡化。然而，高剂量时，空泡化广泛存在，基本影响所有近端小管；而低剂量时，病变呈多灶性分布。以7.3 mmol/kg的剂量经口灌胃给予次氮基三乙酸也诱导了肾近端小管上皮严重的细胞质空泡化。该病变的分布是多灶性的，与7.3 mmol/kg剂量蔗糖所致的病变无法区分。对空泡化小管细胞的电子显微镜检查表明，在NTA和蔗糖处理的动物中，病变均归因于内吞/溶酶体系统的变化。给予NTA或蔗糖的大鼠的空泡化和未空泡化小管细胞中的细胞核、线粒体、高尔基体、内质网以及基底膜的高度卷曲区域看起来均正常。
