Fullem Kiersten R, MacLellan Michelle P, Iriarte Fanny B, Poudel Mousami, Capik Sarah, Dedonder Keith, Bono James L, Harhay Dayna M, Harhay Gregory P, Goss Erica M, Potnis Neha, Minsavage Gerald V, Jones Jeffrey B, Paret Mathews L
Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
Department of Plant Pathology, University of Georgia, Tifton, GA, USA.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006848.
In 2022, a fluorescent bacterial isolate, designated 22-AL-CL-001, was isolated from diseased cantaloupe () foliage collected in Alabama, USA, displaying marginal necrosis and general blighting. Whole-genome sequencing and subsequent comparisons to publicly available bacterial genomes identified isolate USDA-ARS-USMARC-56711, isolated from a clinically healthy calf () in Kansas, USA, as genetically similar to isolate 22-AL-CL-001. The two isolates were then characterized using phenotypic and genetic analyses, including fluorescence on King's medium B; LOPAT reactions; pathogenicity assays on cantaloupe, watermelon and squash plants; 16S rRNA gene sequence analysis; phylogeny based on multi-locus sequence analysis (MLSA) of four housekeeping genes; average nucleotide identity based on blast (ANIb); DNA-DNA hybridization (isDDH) including comparison to the Type Genome Server and biochemical profiling using the Biolog Gen III microplate system. MLSA phylogeny placed both isolates into a single clade within the genus , distant from all included reference strains. ANIb and isDDH analyses definitively identified the isolates as members of the same species (ANIb, 97.25; isDDH, 78.5%), though comparison scores to reference strains were all below the accepted thresholds for species determination. Biolog biochemical profiling, as well as MALDI-TOF mass spectrometry of isolate 22-AL-CL-001, was also unable to identify the isolate as a member of any existing bacterial species. Using a combination of genetic and phenotypic data, we conclude that the two isolates belong to a new species of , for which we propose the name . The specific epithet, , was chosen to represent the location where the type strain 22-AL-CL-001 (NCPPB 4760=LMG 33363) was isolated, Alabama, USA. Taxonomic classification of the two isolates by the Genome Taxonomy Database revealed other previously sequenced bacterial strains that, according to ANI and isDDH results, also belong to .
2022年，从美国阿拉巴马州采集的患病哈密瓜（）叶片中分离出一株荧光细菌菌株，编号为22-AL-CL-001，该叶片表现出叶缘坏死和普遍枯萎症状。通过全基因组测序以及随后与公开可用的细菌基因组进行比较，确定从美国堪萨斯州一头临床健康的小牛（）中分离出的菌株USDA-ARS-USMARC-56711与菌株22-AL-CL-001在基因上相似。然后，使用表型和基因分析对这两个菌株进行了表征，包括在King氏B培养基上的荧光；LOPAT反应；对哈密瓜、西瓜和南瓜植株的致病性测定；16S rRNA基因序列分析；基于四个管家基因的多位点序列分析（MLSA）的系统发育分析；基于blast的平均核苷酸同一性（ANIb）；DNA-DNA杂交（isDDH），包括与类型基因组服务器的比较，以及使用Biolog Gen III微孔板系统进行生化分析。MLSA系统发育分析将这两个菌株置于属内的一个单一分支中，与所有纳入的参考菌株距离较远。ANIb和isDDH分析明确确定这两个菌株为同一物种的成员（ANIb，97.25；isDDH，78.5%），尽管与参考菌株的比较分数均低于物种确定的公认阈值。Biolog生化分析以及菌株22-AL-CL-001的基质辅助激光解吸电离飞行时间质谱分析也无法将该菌株鉴定为任何现有细菌物种的成员。综合遗传和表型数据，我们得出结论，这两个菌株属于属的一个新物种，我们为此提议命名为。种加词被选择用来代表模式菌株22-AL-CL-001（NCPPB 4760 = LMG 33363）的分离地点，即美国阿拉巴马州。基因组分类数据库对这两个菌株的分类学分类揭示了其他先前测序的细菌菌株，根据ANI和isDDH结果，它们也属于。
