Li Guangda, Wang Jiajun, Tian Lei, Ding Mingchao, Liu Yang, Wang Jingfu
Stomatology College of Jiamusi University, Jiamusi, P.R. China.
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, P.R. China.
J Microbiol Biotechnol. 2025 Jul 14;35:e2504034. doi: 10.4014/jmb.2504.04034.
() is one of the most common pathogens associated with oral and maxillofacial space infections (OMSI), significantly impairing patients' quality of life and posing substantial public health risks. Traditional detection methods are usually time-consuming and operationally complex, which limits their applicability for rapid on-site detection. This study introduces a novel, ultrasensitive nucleic acid detection system combining loop-mediated isothermal amplification (LAMP) with Argonaute (Ago) in a one-pot strategy. The combination of LAMP and Ago not only improves specificity and sensitivity but also effectively reduces the risk of aerosol contamination and false positives. The system detects the gene of , enabling rapid detection within 50 min with a limit of detection (LOD) of 10 Colony Forming Units (CFU)/ml for bacterial solutions and 10 ng/μl for plasmids. This system exhibited exceptional specificity by effectively differentiating from other common OSMI bacteria, and was successfully applied to samples from animal OMSI infection models. This innovative one-pot system offers a rapid, reliable, and effective solution for detection, with potential applications in human health and public safety.
（）是与口腔颌面部间隙感染（OMSI）相关的最常见病原体之一，严重损害患者的生活质量并带来重大公共卫生风险。传统检测方法通常耗时且操作复杂，这限制了它们在快速现场检测中的适用性。本研究介绍了一种新颖的超灵敏核酸检测系统，该系统采用一锅法策略将环介导等温扩增（LAMP）与Argonaute（Ago）相结合。LAMP和Ago的结合不仅提高了特异性和灵敏度，还有效降低了气溶胶污染和假阳性的风险。该系统检测（）的基因，能够在50分钟内实现快速检测，对细菌溶液的检测限（LOD）为10个菌落形成单位（CFU）/毫升，对质粒的检测限为10纳克/微升。该系统通过有效区分（）与其他常见的OMSI细菌表现出卓越的特异性，并成功应用于动物OMSI感染模型的样本。这种创新的一锅法系统为（）检测提供了一种快速、可靠且有效的解决方案，在人类健康和公共安全方面具有潜在应用价值。
