Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Biosens Bioelectron. 2024 Jun 15;254:116230. doi: 10.1016/j.bios.2024.116230. Epub 2024 Mar 16.
African swine fever (ASF), which is casued by African swine fever virus (ASFV), is a fatal infectious disease of pigs that results in significant losses to the breeding industry. Therefore, screening and detection are crucial for the control and prevention of the ASFV. Argonaute is a new detection tool that is being extensively used due to its high specificity and programmability. This study reports on a new nucleic acid assay method, termed REPD, which uses recombinase-aided amplification and restriction endonuclease-assisted Pyrococcus furiosus argonaute (PfAgo) detection. One-pot REPD was developed for the detection of ASFV. The one-pot REPD could detect a single copy of ASFV nucleic acid and showed no cross-reactivity with other pathogens. Detection in clinical samples was 100% consistent with the results of real-time PCR analysis. The results showed that the one-pot REPD assay is convenient, sensitive, specific, and potentially adaptable to the detection of ASFV. In summary, this study highlights a novel method that can be employed for the detection of pathogens.
非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）引起的一种致命的猪传染病，会给养殖业造成重大损失。因此，筛选和检测对于控制和预防 ASF 至关重要。Argonaute 是一种新的检测工具，由于其高度特异性和可编程性，正在被广泛使用。本研究报道了一种新的核酸检测方法，称为 REPD，它使用重组酶辅助扩增和内切酶辅助 Pyrococcus furiosus argonaute（PfAgo）检测。开发了一种用于检测 ASFV 的一步法 REPD。该一步法 REPD 可以检测到单个 ASFV 核酸拷贝，与其他病原体没有交叉反应。与实时 PCR 分析的结果完全一致。结果表明，一步法 REPD 检测法方便、灵敏、特异，有潜力适用于 ASFV 的检测。总之，本研究强调了一种新的病原体检测方法。
