Lewalter J, Korallus U
Int Arch Occup Environ Health. 1985;56(3):179-96. doi: 10.1007/BF00396596.
Internal stress of aromatic amines has so far been evaluated by their determination in blood or urine and by the degree of methemoglobin formation. Animal experiments have shown that these materials can form adducts and conjugates with proteins and nucleic acids. Our investigations show that these processes can also occur in human metabolism. For this the degree of such a formation of protein conjugates depends on an individually different potential for acetylation. In a positive sense it influences the magnitude and the rate of renal excretion of aminoaromates and their conjugates and metabolites formed by this metabolism. In contrast, only free nonacetylated aminoaromates can lead to the formation of conjugates with hemoglobin. These aminoaromates or their metabolites can then be detected quantitatively in intact erythrocytes during their lifespan. The degree of this protein conjugate formation correlates inversely with the magnitude of the acetylation potential depending on the availability of free non-acetylated aminoaromates. According to these results a clearer assessment of past stress or the presence of strain can be obtained with Biological Monitoring by a single determination of such hemoglobin adducts rather than by the traditional quantitative determination of aminoaromates or their metabolites in blood and/or urine or the methemoglobin concentration.
迄今为止，芳香胺的内应力是通过测定其在血液或尿液中的含量以及高铁血红蛋白的形成程度来评估的。动物实验表明，这些物质可与蛋白质和核酸形成加合物和共轭物。我们的研究表明，这些过程在人体新陈代谢中也会发生。为此，这种蛋白质共轭物的形成程度取决于个体不同的乙酰化潜力。从积极的方面来看，它会影响氨基芳烃及其共轭物和由这种新陈代谢形成的代谢物的肾脏排泄量和排泄速率。相比之下，只有游离的未乙酰化氨基芳烃才能导致与血红蛋白形成共轭物。然后，这些氨基芳烃或其代谢物在其寿命期间可在完整红细胞中进行定量检测。这种蛋白质共轭物的形成程度与乙酰化潜力的大小呈负相关，这取决于游离未乙酰化氨基芳烃的可用性。根据这些结果，通过单次测定此类血红蛋白加合物进行生物监测，比通过传统的定量测定血液和/或尿液中的氨基芳烃或其代谢物或高铁血红蛋白浓度，能够更清楚地评估过去的应激或压力状况。
