Hassan Doaa Mohamad, Sabra Nourhan Tharwat, Farghaly Maha Eid, Sedeak Ahmed Yahia
Lecturer of Human Anatomy and Embryology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt.
Lecturer of Medical Physiology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt.
J Mol Histol. 2025 Jul 15;56(4):227. doi: 10.1007/s10735-025-10511-6.
There is no treatment for some neurological conditions, like cerebellar ataxia (CA). Moringa Oleifera (MO) has been revealed to have neuroprotective properties, but little is known about how it could protect against CA. In this study, we studied the neuroprotective effects of MO in an animal model of CA induced by 3-acetylpyridine (3-AP), which showed deficits in balance and motor coordination. Although cerebellar neuroinflammatory responses are evident in CA, it is yet unclear how neuroinflammation might influence CA. Here, we investigate whether MO, which has anti-inflammatory, antioxidant, and neuroprotective qualities, can help with cerebellar neurodegeneration and locomotor activity deficits. We divided 24 adult male rats into four equal groups. The control group received saline orally, the MO group received MO extract orally, the 3-AP group was injected with 3-AP, and the 3-AP + MO combined group received both 3-AP and MO for four weeks. The animals underwent a motor coordination test on the experiment's first and last days. At the end of the experiment, the animals were euthanized, the cerebellums were dissected, and they were then subjected to standard biochemical, histological, and immunohistochemical studies. The combined group showed remarkable improvement in the CA. The cerebellar neurodegeneration and declination of locomotor activity had improved. Our findings imply that MO may protect against the CA degenerative condition and improve cerebellar function.
对于某些神经系统疾病，如小脑共济失调（CA），目前尚无治疗方法。辣木（MO）已被证实具有神经保护特性，但对于其如何预防CA却知之甚少。在本研究中，我们研究了MO在由3 - 乙酰吡啶（3 - AP）诱导的CA动物模型中的神经保护作用，该模型表现出平衡和运动协调能力缺陷。虽然在CA中明显存在小脑神经炎症反应，但神经炎症如何影响CA尚不清楚。在此，我们研究具有抗炎、抗氧化和神经保护特性的MO是否有助于改善小脑神经退行性变和运动活动缺陷。我们将24只成年雄性大鼠平均分为四组。对照组口服生理盐水，MO组口服MO提取物，3 - AP组注射3 - AP，3 - AP + MO联合组同时接受3 - AP和MO，持续四周。在实验的第一天和最后一天对动物进行运动协调测试。实验结束时，对动物实施安乐死，解剖小脑，然后进行标准的生化、组织学和免疫组织化学研究。联合组在CA方面显示出显著改善。小脑神经退行性变和运动活动衰退得到改善。我们的研究结果表明，MO可能预防CA退行性疾病并改善小脑功能。
