Angen Øystein, Karstensen Kasper Thystrup, Vilaró Anna, Cavaco Lina Maria, Langford Paul R, Sauce Lorenzo Jose Fraile, Migura-Garcia Lourdes, Salomonsen Charlotte Mark, Li Yanwen, Bossé Janine T
Department of Bacteria, Parasites, and Fungi, Statens Serum Institut (SSI), Copenhagen, Denmark.
Grup de Sanejament Porcí (GSP), Lleida, Spain.
Microb Genom. 2025 Jul;11(7). doi: 10.1099/mgen.0.001434.
Serovar detector is a new bioinformatic tool for determining the serovar of using whole genome sequencing. The composition of genes of isolates is compared to those of the serovar reference strains, and the outcome is determined both by the number of common genes and the similarities between the homologous genes. The validation of the bioinformatic tool utilized a broad collection of 732 isolates, including representatives from all described serovars. The isolates included had been characterized by conventional serotyping, PCR tests or different bioinformatic tools. The collection also includes isolates that have been difficult to allocate to a serovar using serology to test the performance of the Serovar detector when potential new varieties or combinations of genes are present. Out of the 732 isolates included in the investigation, only 36 isolates (4.9%) could not be allocated to the 19 recognized serovars. The validation showed that the Serovar detector is a robust method for determining the serovar of an isolate and a valuable tool for further characterization of the genetic heterogeneity both within serovars and within the species.
血清型检测器是一种利用全基因组测序来确定血清型的新型生物信息学工具。将分离株的基因组成与血清型参考菌株的基因组成进行比较，结果由共同基因的数量和同源基因之间的相似性决定。该生物信息学工具的验证使用了732株分离株的广泛集合，包括所有已描述血清型的代表菌株。所纳入的分离株已通过传统血清分型、聚合酶链反应（PCR）检测或不同的生物信息学工具进行了鉴定。该集合还包括那些使用血清学难以分配到血清型的分离株，以测试血清型检测器在存在潜在新基因变体或基因组合时的性能。在调查所纳入的732株分离株中，只有36株（4.9%）无法分配到19种公认的血清型中。验证表明，血清型检测器是确定分离株血清型的一种可靠方法，也是进一步表征血清型内和物种内遗传异质性的有价值工具。
