de Jong-Brink M, de Wit A, Kraal G, Boer H H
Cell Tissue Res. 1976 Aug 20;171(2):195-219. doi: 10.1007/BF00219406.
In the freshwater snail Biomphalaria glabrata the formation and composition of yolk granules and the role of the follicle cells were studied by histochemical and electron microscopical techniques. The rough endoplasmic reticulum and the Golgi apparatus appeared to be involved in yolk formation, which is a continuous process throughout oogenesis. From the very beginning of yolk formation two main types of yolk granules were distinguished morphologically. However, with histochemical and enzyme cytochemical methods no differences were observed between these types. The granules acquire lysosomal enzymes after oviposition, indicating that their main function is probably digestion of perivitelline fluid, which contains nutrients for the developing embryo. Yolk formation and the activity of the follicle cells were studied in successive stages of oogenesis by quantitative electron microscopy. The data strongly suggest that the follicle cells are involved in the formation of the follicular cavity and hence in the ovulation process.
通过组织化学和电子显微镜技术，对淡水螺光滑双脐螺卵黄颗粒的形成、组成以及卵泡细胞的作用进行了研究。粗面内质网和高尔基体似乎参与了卵黄形成过程，而卵黄形成在整个卵子发生过程中是一个持续的过程。从卵黄形成一开始，就在形态上区分出两种主要类型的卵黄颗粒。然而，用组织化学和酶细胞化学方法未观察到这些类型之间的差异。产卵后，颗粒获得溶酶体酶，这表明它们的主要功能可能是消化卵周液，卵周液含有发育中胚胎所需的营养物质。通过定量电子显微镜对卵子发生的连续阶段中卵黄形成和卵泡细胞的活性进行了研究。数据有力地表明，卵泡细胞参与卵泡腔的形成，从而参与排卵过程。
