Lin P S, Wallach D F, Tsai S
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2492-6. doi: 10.1073/pnas.70.9.2492.
Temperature-induced variations in the surface morphology of cultured lymphocytes were evaluated by scanning electron microscopy. At 25-37 degrees the cells' surfaces are largely obscured by numerous undulating microvilli of various lengths but uniform diameter. Temperature changes alter the number of microvilli, their lengths, diameters, distribution, branching, and fusing. Typically, chilling to 0-4 degrees markedly reduces the number of microvilli and increases the diameter of the survivors in a reversible process. In contrast, heating the cells to about 45 degrees rapidly and irreversibly transforms the ordinarily smooth membrane surface into one with a "cobblestone" morphology. At the same time most microvilli disappear and the few that remain clump into a cap. The data suggest that the low-temperature effects reflect a change in the physical state of membrane lipids, while the high-temperature alterations represent thermotropic protein transitions.
通过扫描电子显微镜评估温度诱导培养淋巴细胞表面形态的变化。在25至37摄氏度时，细胞表面大部分被众多长短不一但直径均匀的起伏微绒毛所遮盖。温度变化会改变微绒毛的数量、长度、直径、分布、分支和融合情况。通常，冷却至0至4摄氏度会显著减少微绒毛的数量，并在一个可逆过程中增加留存微绒毛的直径。相比之下，将细胞加热至约45摄氏度会迅速且不可逆地将通常光滑的膜表面转变为具有“鹅卵石”形态的表面。与此同时，大多数微绒毛消失，少数留存的微绒毛聚集成帽状。数据表明，低温效应反映了膜脂物理状态的变化，而高温改变则代表了热致性蛋白质转变。
