用神经氨酸酶 - 半乳糖氧化酶处理后，美洲商陆丝裂原对慢性淋巴细胞白血病细胞的刺激作用。
Stimulation of chronic lymphatic leukaemia cells by pokeweed mitogen after treatment with neuraminidase-galactose oxidase.
作者信息
Zafar M N, Pittman S, Cherchi M, Catovsky D
出版信息
Clin Exp Immunol. 1981 Apr;44(1):124-8.
CLL lymphocytes gave a low response upon stimulation with PHA or PWM in 3-day cultures. However, after treatment with neuraminidase-galactose oxidase (NGO), in the presence of PWM, CLL lymphocytes transformed into blasts and incorporated 3H-thymidine in 3-day cultures. This response of CLL lymphocytes was similar to that given by normal lymphocytes to PWM in 3-day cultures. The best stimulation of CLL lymphocytes was achieved when conditioned medium (CM) from normal T lymphocytes was present in PWM cultures. Purified B lymphocytes from CLL (T lymphocytes and monocytes removed) did not respond to PHA or PWM. However, after NGO treatment these cells were stimulated by PWM, but only in the presence of CM. PHA failed to stimulate NGO-treated CLL lymphocytes or purified B lymphocytes. This study shows that CLL lymphocytes, which usually fail to respond to mitogens, can be stimulated by PWM to proliferate after treatment with neuraminidase-galactose oxidase (NGO). This technique of B cell stimulation has been found useful in cytogenetic studies of B cell proliferative disorders.
在3天的培养中，慢性淋巴细胞白血病（CLL）淋巴细胞在用植物血凝素（PHA）或美洲商陆有丝分裂原（PWM）刺激时反应较弱。然而，在用神经氨酸酶 - 半乳糖氧化酶（NGO）处理后，在PWM存在的情况下，CLL淋巴细胞在3天的培养中转化为母细胞并掺入3H - 胸腺嘧啶核苷。CLL淋巴细胞的这种反应类似于正常淋巴细胞在3天培养中对PWM的反应。当PWM培养物中存在来自正常T淋巴细胞的条件培养基（CM）时，对CLL淋巴细胞的刺激最佳。从CLL中纯化的B淋巴细胞（去除T淋巴细胞和单核细胞）对PHA或PWM无反应。然而，在NGO处理后，这些细胞被PWM刺激，但仅在CM存在的情况下。PHA未能刺激经NGO处理的CLL淋巴细胞或纯化的B淋巴细胞。这项研究表明，通常对有丝分裂原无反应的CLL淋巴细胞在用神经氨酸酶 - 半乳糖氧化酶（NGO）处理后可被PWM刺激增殖。已发现这种B细胞刺激技术在B细胞增殖性疾病的细胞遗传学研究中有用。
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