照射野外的中子俘获增强质子束疗法：体外实验

Neutron capture-enhanced proton beam therapy outside the irradiation field: in vitro experiments.

作者信息

Shiba Shintaro, Shimo Takahiro, Yamanaka Masashi, Matsumoto Kazuki, Yamano Akihiro, Nitta Kazunori, Sakai Makoto, Ohno Tatsuya, Tokuuye Koichi, Omura Motoko

机构信息

Department of Radiation Oncology, Shonan Kamakura General Hospital, 1370-1, Okamoto, 247-8533, Kamakura, Kanagawa, Japan.

Department of Radiation Oncology, Gunma University Graduate School of Medicine, Gunma, Japan.

出版信息

BMC Res Notes. 2025 Jul 15;18(1):297. doi: 10.1186/s13104-025-07362-5.

DOI:10.1186/s13104-025-07362-5

PMID:40665442

Abstract

OBJECTIVE

To evaluate the cell-killing effects of neutron capture-enhanced particle therapy (NCEPT) outside the proton beam irradiation (PBI) field.

RESULTS

Human osteosarcoma cells (MG63) were divided into control and NCEPT groups. Four hours before irradiation, the NCEPT group was exposed to B-boronophenylalanine. PBI was performed by targeting a gel bolus, with tissue culture plates placed outside the PBI field adjacent to the gel bolus. The bolus was irradiated with 12, 36, or 72 Gy. Cell survival fractions were calculated for wells adjacent to the bolus. Furthermore, the neutron fluence generated by PBI in the tissue culture plates was calculated using a Monte Carlo simulation. After 12, 36, and 72 Gy bolus irradiation, the cell survival rates in the control group were 103%, 95%, and 95%, respectively, whereas those in the NCEPT group were 84%, 75%, and 51%, respectively. The cell survival fraction in the NCEPT group was significantly lower than that in the control group (P < 0.01). The mean neutron fluence in the wells adjacent to the bolus was 1.24 × 10 (1/cm) at 72 Gy irradiation. Although NCEPT enhanced cell-killing outside the PBI field, the magnitude of this effect was modest.

摘要

目的

评估中子俘获增强粒子疗法（NCEPT）在质子束照射（PBI）野之外的细胞杀伤效果。

结果

将人骨肉瘤细胞（MG63）分为对照组和NCEPT组。照射前4小时，NCEPT组细胞暴露于B - 硼苯基丙氨酸。通过靶向凝胶体模进行PBI，将组织培养板放置在PBI野之外且与凝胶体模相邻。分别用12、36或72 Gy照射体模。计算与体模相邻孔中的细胞存活分数。此外，使用蒙特卡罗模拟计算PBI在组织培养板中产生的中子注量。在体模接受12、36和72 Gy照射后，对照组的细胞存活率分别为103%、95%和95%，而NCEPT组分别为84%、75%和51%。NCEPT组的细胞存活分数显著低于对照组（P < 0.01）。在72 Gy照射时，与体模相邻孔中的平均中子注量为1.24×10（1/cm）。尽管NCEPT增强了PBI野之外的细胞杀伤作用，但这种作用的程度适中。