伊朗阿瓦士废水样本中JC多瘤病毒的定量分析。

The quantification of JC polyomavirus in wastewater samples in Ahvaz, Iran.

作者信息

Bahrami Parvaneh, Takdastan Afshin, Makvandi Manoochehr, Jorfi Sahand, Karimi Baba Ahmadi Mohammad, Neisi Abdolkazem

机构信息

Student Research Committee, Department of Environmental Health Engineering, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Environmental Technologies Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Int J Environ Health Res. 2025 Jul 15:1-13. doi: 10.1080/09603123.2025.2515231.

DOI:10.1080/09603123.2025.2515231

PMID:40665652

Abstract

The presence of human JC polyomavirus (JCPyV) in wastewater serves as a notable marker of human fecal and urine of infected individuals. Therefore, this study investigated the occurrence of JCPyV in wastewater samples from diverse sites across Ahvaz city. To this aim, 60 wastewater samples were collected from different areas. The samples were concentrated using polyethylene glycol 6000 prior to isolating the viral nucleic acids. Circulating JCPyV genotypes in the wastewater samples were determined. Furthermore, nested PCR was performed to amplify the non-coding control region (NCCR) of the JCPyV genome to assess for the presence of nucleotide mutations within this region. JCPyV DNA in wastewater samples was quantified using SYBR Green real-time PCR (qPCR). The analysis showed 16/60 (26.6%) samples tested positive for both JCPyV VP1 and NCCR genomic regions. The analysis of phylogenetic tree revealed that JCPyV genotype 3 is circulating in this region. The outcomes of sequencing NCCR-JCPyV blocks isolates were found to be archetype. However, the occurrence of nucleotide mutations was observed in NCCR sections "c", "d", and "f" blocks. The concentration of JCPyV DNA in 16/60 (26.6%) positive wastewater samples ranged from 3.71 × 103 to 1.31 × 105 genomic copies per liter.

摘要

废水中人类 JC 多瘤病毒（JCPyV）的存在是受感染个体人类粪便和尿液的显著标志物。因此，本研究调查了阿瓦士市不同地点废水样本中 JCPyV 的出现情况。为此，从不同区域收集了 60 份废水样本。在分离病毒核酸之前，使用聚乙二醇 6000 对样本进行浓缩。测定了废水样本中循环的 JCPyV 基因型。此外，进行巢式 PCR 以扩增 JCPyV 基因组的非编码控制区（NCCR），以评估该区域内核苷酸突变的存在情况。使用 SYBR Green 实时 PCR（qPCR）对废水样本中的 JCPyV DNA 进行定量。分析显示，60 份样本中有 16 份（26.6%）在 JCPyV VP1 和 NCCR 基因组区域检测均呈阳性。系统发育树分析表明，JCPyV 基因型 3 在该区域循环。NCCR-JCPyV 区段分离株的测序结果为原型。然而，在 NCCR 区段的“c”、“d”和“f”块中观察到了核苷酸突变。16 份（26.6%）阳性废水样本中 JCPyV DNA 的浓度范围为每升 3.71×10³至 1.31×10⁵个基因组拷贝。