Truchet G, Debellé F, Vasse J, Terzaghi B, Garnerone A M, Rosenberg C, Batut J, Maillet F, Dénarié J
J Bacteriol. 1985 Dec;164(3):1200-10. doi: 10.1128/jb.164.3.1200-1210.1985.
In Rhizobium meliloti 2011 nodulation genes (nod) required to nodulate specifically alfalfa are located on a pSym megaplasmid. Nod- derivatives carrying large pSym deletions were isolated. By complementation of these strains with in vivo- and in vitro-constructed episomes containing pSym of sequences and introduction of these episomes into Agrobacterium tumefaciens, we show (i) that from a region of pSym of about 360 kilobases, genes required for specific alfalfa nodulation are clustered in a DNA fragment of less than 30 kilobases and (ii) that a nod region located between nifHDK and the common nod genes is absolutely required for alfalfa nodulation and controls the specificity of root hair curling and nodule organogenesis initiation.
在苜蓿中华根瘤菌2011中，特异性结瘤苜蓿所需的结瘤基因（nod）位于共生大质粒（pSym）上。分离出携带pSym大片段缺失的nod-衍生物。通过用含有pSym序列的体内和体外构建的附加体对这些菌株进行互补，并将这些附加体导入根癌农杆菌，我们发现：（i）在约360千碱基的pSym区域中，特异性苜蓿结瘤所需的基因聚集在小于30千碱基的DNA片段中；（ii）位于nifHDK和共同结瘤基因之间的一个nod区域对于苜蓿结瘤是绝对必需的，并且控制根毛卷曲和根瘤器官发生起始的特异性。
