苜蓿根瘤菌的两个基因簇编码早期必需的结瘤功能，第三个基因簇影响结瘤效率。
Two gene clusters of Rhizobium meliloti code for early essential nodulation functions and a third influences nodulation efficiency.
作者信息
Putnoky P, Kondorosi A
出版信息
J Bacteriol. 1986 Sep;167(3):881-7. doi: 10.1128/jb.167.3.881-887.1986.
Abstract
A pLAFR1 cosmid clone (pPP346) carrying the nodulation region of the symbiotic plasmid pRme41b was isolated from a gene library of Rhizobium meliloti 41 by direct complementation of a Nod- deletion mutant of R. meliloti. Agrobacterium tumefaciens and Rhizobium species containing pPP346 were able to form ineffective nodules on alfalfa. The 24-kilobase insert in pPP346 carries both the common nodulation genes and genes involved in host specificity of nodulation. It was shown that these two regions are essential and sufficient to determine the early events in nodulation. A new DNA region influencing the kinetics and efficiency of nodulation was also localized on the symbiotic megaplasmid at the right side of the nif genes.
摘要
通过苜蓿根瘤菌Nod-缺失突变体的直接互补，从苜蓿根瘤菌41的基因文库中分离出一个携带共生质粒pRme41b结瘤区域的pLAFR1黏粒克隆（pPP346）。含有pPP346的根癌农杆菌和根瘤菌能够在苜蓿上形成无效根瘤。pPP346中24千碱基的插入片段既携带共同结瘤基因，也携带参与结瘤宿主特异性的基因。结果表明，这两个区域对于确定结瘤早期事件是必不可少且足够的。一个影响结瘤动力学和效率的新DNA区域也定位在固氮基因右侧的共生大质粒上。
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Two gene clusters of Rhizobium meliloti code for early essential nodulation functions and a third influences nodulation efficiency.苜蓿根瘤菌的两个基因簇编码早期必需的结瘤功能，第三个基因簇影响结瘤效率。
J Bacteriol. 1986 Sep;167(3):881-7. doi: 10.1128/jb.167.3.881-887.1986.
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引用本文的文献
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Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti.控制苜蓿根瘤菌共生早期和晚期功能的基因位于一个大质粒上。
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Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti.苜蓿中华根瘤菌高分子量质粒上结瘤和固氮基因的定位
Mol Gen Genet. 1981;184(2):318-25. doi: 10.1007/BF00272925.
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Morphology of root nodules and nodule-like structures formed by Rhizobium and Agrobacterium strains containing a Rhizobium meliloti megaplasmid.含有苜蓿根瘤菌大质粒的根瘤菌和农杆菌菌株形成的根瘤及类根瘤结构的形态学
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