采用柱前丹磺酰氯衍生化和荧光检测的液相色谱法用于生物样品中吗啡的测定。

Liquid chromatography with pre-column dansyl derivatisation and fluorimetric detection applied to the assay of morphine in biological samples.

作者信息

Tagliaro F, Frigerio A, Dorizzi R, Lubli G, Marigo M

出版信息

J Chromatogr. 1985 Aug 23;330(2):323-31. doi: 10.1016/s0021-9673(01)81989-0.

DOI:10.1016/s0021-9673(01)81989-0

Abstract

A simple method employing pre-column dansylation and liquid chromatography is proposed for a very sensitive and specific assay of morphine in biological samples. Nalorphine is used as an internal standard. The detection limit is 0.2 picomol of injected morphine. In the assay of human sera spiked with 150 nmol/l, the intra- and inter-assay coefficients of variation were 3.7% (n = 10) and 4.5% (n = 10), respectively. No interferences were observed from more than 70 opiate and non-opiate drugs. Urine, plasma and total blood were assayed, using different extraction methods, with negligible interference from coextractives.

摘要

提出了一种采用柱前丹磺酰化和液相色谱法的简单方法，用于生物样品中吗啡的高灵敏度和特异性测定。烯丙吗啡用作内标。检测限为注入吗啡0.2皮摩尔。在加标浓度为150纳摩尔/升的人血清测定中，批内和批间变异系数分别为3.7%（n = 10）和4.5%（n = 10）。70多种阿片类和非阿片类药物均未观察到干扰。使用不同的提取方法对尿液、血浆和全血进行了测定，共提取物的干扰可忽略不计。