Aletaha Mansoor, Heidarzadeh Siamak, Rahimi Foroushani Abbas, Soltan Dallal Mohammad Mehdi
Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Microb Pathog. 2025 Oct;207:107901. doi: 10.1016/j.micpath.2025.107901. Epub 2025 Jul 14.
Yersinia enterocolitica is a common pathogen between humans and animals and is one of the important bacterial pathogens causing gastrointestinal diseases worldwide. The aim of this study is to determine the prevalence of Y. enterocolitica in diarrhea of children under 5 years of age and to identify the isolates in terms of bioserotypes, virulence factors, antimicrobial sensitivity, and genetic diversity. During 2022-2023, 1092 diarrhea samples were collected from hospitals and analyzed to detect and isolate Y. enterocolitica. Biotype and serotype were determined by biochemical and agglutination methods. Six virulence genes were investigated by the PCR method. Antimicrobial resistance was tested by the disk diffusion method. Heterogeneity was done by the PCR ribotyping method. The prevalence of Y. enterocolitica was detected in 2.6 %. Bioserotypes 1A/O:8, 1A/O:NI, 1B/O:8 and 1B/O:NI were identified; most of the isolates were in the age group of less than one year. Abdominal pain and fever were the most common clinical symptoms in 1B/O:8. There was a significant relationship between the cold season and isolated bioserotypes (P = 0.0001). In biotype 1B isolates, the most common virulence genes were ystA, myfA, virF, ail, and inv; and in biotype 1A, ystB and inv. All isolates were sensitive to 14 antibiotics. Resistance to ampicillin was 100 %. High resistance to nitrofurantoin and ceftazidime was observed. Twenty-five isolates of Y. enterocolitica were analyzed by PCR ribotyping method, which identified 3 SR. The outbreak of highly pathogenic Y. enterocolitica 1B/O:8 in Iran may be a serious threat to the public health of Iran.
小肠结肠炎耶尔森菌是人和动物之间常见的病原体，是全球引起胃肠道疾病的重要细菌病原体之一。本研究的目的是确定5岁以下儿童腹泻中小肠结肠炎耶尔森菌的流行情况，并从生物血清型、毒力因子、抗菌敏感性和遗传多样性方面鉴定分离株。在2022 - 2023年期间，从医院收集了1092份腹泻样本，进行分析以检测和分离小肠结肠炎耶尔森菌。通过生化和凝集方法确定生物型和血清型。通过PCR方法研究6个毒力基因。通过纸片扩散法测试抗菌耐药性。通过PCR核糖体分型方法进行异质性分析。检测到小肠结肠炎耶尔森菌的流行率为2.6%。鉴定出生物血清型1A/O:8、1A/O:NI、1B/O:8和1B/O:NI；大多数分离株来自1岁以下年龄组。腹痛和发热是1B/O:8中最常见的临床症状。寒冷季节与分离的生物血清型之间存在显著关系（P = 0.0001）。在1B生物型分离株中，最常见的毒力基因是ystA、myfA、virF、ail和inv；在1A生物型中，是ystB和inv。所有分离株对14种抗生素敏感。对氨苄西林的耐药率为100%。观察到对呋喃妥因和头孢他啶的高耐药性。通过PCR核糖体分型方法分析了25株小肠结肠炎耶尔森菌，鉴定出3个核糖体分型。伊朗高致病性小肠结肠炎耶尔森菌1B/O:8的暴发可能对伊朗公众健康构成严重威胁。
