Pan Bingbing, Chen Fengting, Shen Siyan, Jiang Yu, Yang Ying, Wang Jia, Liu Yanjuan, Wei Lai, Liu Jitong, Kong Gaoyin, He Xuan, Guo Bing
Department of Anesthesiology, Clinical Research Center for Anesthesiology of ERAS in Hunan Province, Hunan Provincial People's Hospital and The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, Hunan, China.
The First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha, 410005, Hunan, China.
Sci Rep. 2025 Jul 16;15(1):25861. doi: 10.1038/s41598-025-11545-2.
Sepsis, a severe infectious disease causing multiple organ dysfunction, requires further exploration of its pathomechanisms and therapeutic options. Hydroxysafflor Yellow A (HYSA), extracted from Carthamus tinctorius L, has shown anti-inflammatory and antioxidant properties effective in treating sepsis, though its precise mechanism remains unclear. In this study, a cecum ligation puncture (CLP) model was used to evaluate HYSA's effects on sepsis-induced organ injury. HYSA (300 mg/kg) was administered intraperitoneally 4 h post-CLP. Results showed that HYSA inhibited the inflammatory response and improved pathological scores in the liver, lungs, and kidneys. Metabolomics analysis identified key metabolites and pathways influenced by HYSA, including nicotinate and nicotinamide metabolism and glycine/serine metabolism. Network pharmacology and molecular docking identified JAK2/STAT1 as potential targets, with Western blotting confirmed that HYSA inhibits JAK2/STAT1 phosphorylation. These findings suggest that HYSA protects against sepsis-induced organ injury by regulating metabolic networks and modulating the JAK2/STAT1 pathway.
脓毒症是一种导致多器官功能障碍的严重传染病，需要进一步探索其发病机制和治疗方案。从红花中提取的羟基红花黄色素A（HYSA）已显示出抗炎和抗氧化特性，对治疗脓毒症有效，但其确切机制仍不清楚。在本研究中，采用盲肠结扎穿刺（CLP）模型评估HYSA对脓毒症诱导的器官损伤的影响。在CLP后4小时腹腔注射HYSA（300mg/kg）。结果表明，HYSA抑制了炎症反应，改善了肝脏、肺和肾脏的病理评分。代谢组学分析确定了受HYSA影响的关键代谢物和途径，包括烟酸和烟酰胺代谢以及甘氨酸/丝氨酸代谢。网络药理学和分子对接确定JAK2/STAT1为潜在靶点，蛋白质免疫印迹法证实HYSA抑制JAK2/STAT1磷酸化。这些发现表明，HYSA通过调节代谢网络和调节JAK2/STAT1途径来预防脓毒症诱导的器官损伤。
