Zhao Yunlong, Zhang Jiabin, Xia Qianqian, Yang Jinyu, Chen Daichao, Xia Yu, Yu Hao, Shen Qiuyue, Liang Dongdong, Hou Xinlin, Zhang Jue
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
Children's Medical Center, Peking University First Hospital, Beijing, China.
Pediatr Res. 2025 Jul 16. doi: 10.1038/s41390-025-04275-8.
Hypoxic-ischemic encephalopathy (HIE) leads to poor neurological outcomes without timely treatment, yet early identification remains challenging. While cerebral blood flow (CBF) changes relate to HIE, specific early CBF or cerebral venous drainage (CVD) patterns are unclear. Recently, the advanced multi-angle plane wave high-frequency ultrafast Doppler (HF-μDoppler) imaging has shown promise for neonatal CBF/CVD assessment, aiding early diagnosis and intervention of HIE.
This study modified the Rice-Vannucci model to enable real-time observation of CBF/CVD changes during HIE. Using HF-μDoppler, we evaluated its feasibility and performance in assessing CBF/CVD under different conditions and stages of HIE, and explored specific early CBF/CVD alterations during HIE onset.
This study validated the feasibility and sensitivity of HF-μDoppler for detecting CBF/CVD anomalies in HIE rats. We identified early-stage HIE-specific retrograde perfusion of the transverse sinus (TS) contralateral to carotid ligation, with reduced velocity and increased diameter on both sides compared to baseline. The contralateral TS showed lower velocity and smaller diameter than the ipsilateral side, with potential explanations discussed.
HF-μDoppler is a promising imaging modality for reliable HIE assessment. The observed retrograde perfusion of TS may serve as a sensitive early biomarker for accurate HIE detection, supporting earlier clinical diagnosis and treatment.
HF-μDoppler can be used for CBF/CVD evaluation in HIE newborn rats. HF-μDoppler can detect sensitive biomarkers in the early stages of HIE. Transverse sinus' retrograde perfusion specifically occurs in HIE' early stages.
缺氧缺血性脑病（HIE）若不及时治疗会导致不良神经预后，但早期识别仍具有挑战性。虽然脑血流量（CBF）变化与HIE相关，但具体的早期CBF或脑静脉引流（CVD）模式尚不清楚。最近，先进的多角度平面波高频超快多普勒（HF-μDoppler）成像在新生儿CBF/CVD评估方面显示出前景，有助于HIE的早期诊断和干预。
本研究对Rice-Vannucci模型进行了改良，以实时观察HIE期间CBF/CVD的变化。使用HF-μDoppler，我们评估了其在评估HIE不同条件和阶段下CBF/CVD的可行性和性能，并探索了HIE发作期间早期特定的CBF/CVD改变。
本研究验证了HF-μDoppler检测HIE大鼠CBF/CVD异常的可行性和敏感性。我们发现了HIE早期特异性的、与颈动脉结扎对侧的横窦（TS）逆行灌注，与基线相比，两侧速度降低、直径增加。对侧TS的速度低于同侧，直径小于同侧，并讨论了可能的解释。
HF-μDoppler是一种有前景的成像方式，可用于可靠的HIE评估。观察到的TS逆行灌注可能作为准确检测HIE的敏感早期生物标志物，支持更早的临床诊断和治疗。
HF-μDoppler可用于HIE新生大鼠的CBF/CVD评估。HF-μDoppler可在HIE早期检测到敏感生物标志物。横窦逆行灌注特异性发生在HIE早期。
