从返回英国的旅行者中分离出携带blaPER-7的高度耐药霍乱弧菌。

Highly drug-resistant Vibrio cholerae harbouring blaPER-7 isolated from travellers returning to England.

作者信息

Nair Satheesh, Barker Clare R, Patel Vineet, Poh Ching-Ying, Greig David R, Olonade Israel, Ribeca Paolo, Jenkins Claire

机构信息

Gastrointestinal Bacteria Reference Unit, UK Health Security Agency, London NW9 5HT, UK.

NIHR Health Protection Research Unit in Gastrointestinal Infections, University of East Anglia, Norwich, UK.

出版信息

J Antimicrob Chemother. 2025 Sep 3;80(9):2428-2432. doi: 10.1093/jac/dkaf232.

DOI:10.1093/jac/dkaf232

PMID:40671289

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12404714/

Abstract

OBJECTIVES

The current, seventh cholera pandemic (7PET) is notably different from the previous six, owing to its rapid global spread and increased transmission of antimicrobial resistance. The global emergence of MDR Vibrio cholerae is a public health concern. We aimed to interrogate WGS data from V. cholerae isolates referred to the UK Health Security Agency (formerly Public Health England, PHE) for the presence of blaPER-7 encoding resistance to the extended-spectrum cephalosporins.

METHODS

We reviewed 161 genomes of V. cholerae isolated between 2019 and 2024 from travellers returning to the UK, screening this dataset for resistance to the extended-spectrum cephalosporins encoded by blaPER-7.

RESULTS

We identified 3/51 V. cholerae O1 ST69 isolates and 1/110 non-O1 isolate belonging to ST555, harbouring blaPER-7 alongside mph(A), encoding azithromycin resistance. Long-read sequencing confirmed that both these genes were on a YemVchMDR1 element, that also possessed an MDR island carrying genes encoding resistance to aminoglycosides, sulphonamides and florfenicol. This element was located on an IncC plasmid in the ST69 O1 isolates but had inserted into the chromosome of the non-O1 isolate.

CONCLUSIONS

Public health institutions have the ability and responsibility to monitor the burden and spread of highly drug-resistant V. cholerae via passive surveillance, informing clinical guidance, empirical treatment and travel advice.

摘要

目的

当前的第七次霍乱大流行（7PET）与前六次显著不同，因其在全球迅速传播且抗菌药物耐药性传播增加。多重耐药霍乱弧菌在全球出现是一个公共卫生问题。我们旨在调查提交给英国卫生安全局（原英国公共卫生署，PHE）的霍乱弧菌分离株的全基因组测序（WGS）数据，以检测是否存在编码对超广谱头孢菌素耐药的blaPER-7。

方法

我们回顾了2019年至2024年间从返回英国的旅行者中分离出的161株霍乱弧菌的基因组，在该数据集中筛查由blaPER-7编码的对超广谱头孢菌素的耐药性。

结果

我们在51株霍乱弧菌O1 ST69分离株中鉴定出3株，在110株属于ST555的非O1分离株中鉴定出1株，它们携带blaPER-7以及编码阿奇霉素耐药性的mph(A)。长读长测序证实这两个基因都位于YemVchMDR1元件上，该元件还拥有一个多重耐药岛，携带编码对氨基糖苷类、磺胺类和氟苯尼考耐药的基因。该元件在ST69 O1分离株中位于IncC质粒上，但已插入非O1分离株的染色体中。