通过聚焦过程中电场的重复扫描对合成载体两性电解质进行表征。

Characterization of synthetic carrier ampholytes by repetitive scanning of the electric field during focusing.

作者信息

Thormann W, Egen N B, Mosher R A, Bier M

出版信息

J Biochem Biophys Methods. 1985 Oct;11(4-5):287-93. doi: 10.1016/0165-022x(85)90010-7.

Abstract

This paper reports the utilization of a potential gradient array detector for monitoring the dynamics of the electric field during isoelectric focusing. Transient and steady state electric field profiles are presented for synthetic carrier ampholyte mixtures with a wide (approximately 3-10) pH range. Two available commercial products (Ampholine and Pharmalyte) and a laboratory synthesized mixture (PEHA ampholytes) are compared. The formation of conductivity gaps and their migration toward the cathode in extended experiments (cathodic drift) can be visualized with this system.

摘要

本文报道了利用电位梯度阵列检测器监测等电聚焦过程中电场的动态变化。给出了具有宽（约3 - 10）pH范围的合成载体两性电解质混合物的瞬态和稳态电场分布图。比较了两种市售产品（两性电解质和Pharmalyte）以及一种实验室合成混合物（PEHA两性电解质）。利用该系统可以观察到在长时间实验中（阴极漂移）电导率间隙的形成及其向阴极的迁移。