Dai Chunyan, Li Minghua, Pu Rongfeng, Lin Yameng, Liu Hualin, Liu Yuan, Cui Xiuming, Liao Peiran, Yang Ye
School of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province, Kunming, China.
Front Microbiol. 2025 Jul 2;16:1631869. doi: 10.3389/fmicb.2025.1631869. eCollection 2025.
, a perennial medicinal plant, suffers from severe continuous cropping obstacles. Chloropicrin (CP) as a soil fumigant can be used to effectively mitigate continuous cropping obstacles. Mulch film application plays a crucial role in enhancing the effectiveness of CP soil fumigation. However, the effects of mulch film application on soil microorganisms and quality of , as well as underlying mechanisms, are unclear.
To investigate the effect of cover thickness on fumigation efficacy following CP treatment, this study compared soil temperature, humidity, CP residue, microbial diversity, and crop parameters under soil covered with films of 0.06 mm (6S) and 0.08 mm (8S) thickness after CP fumigation.
The 6S film showed less degradation, higher transparency, tensile strength, and elongation at break than 8S. Soil temperature (ST) was lower, and soil water content (SWC) higher under 6S mulch film application with CP fumigation (F6S) compared to 8S (F8S). On the 14th day of fumigation, the CP content of F6S treatment was 28.97% higher than that of F8S treatment. F6S increased beneficial microbial phyla and genera such as , , and , and reduced harmful and Nectriaceae more effectively than F8S. Beneficial bacteria OTUs were significantly correlated with mulch thickness (MT), ST, and SWC. In addition, the F6S maintained the rhizosphere microbial diversity balance and inhibited the accumulation of pathogens ( and ), leading to a high seedling survival rate. The above changes further promoted the accumulation of biomass and saponins in . Overall, F6S treatment improved fumigation efficacy and the yield and quality of , making it a strategic solution to regenerate the soil health, quality, and production of functional root crops facing continuous cropping obstacles.
作为一种多年生药用植物，遭受严重的连作障碍。氯化苦（CP）作为一种土壤熏蒸剂可用于有效减轻连作障碍。地膜覆盖在提高CP土壤熏蒸效果方面起着关键作用。然而，地膜覆盖对土壤微生物和药材品质的影响及其潜在机制尚不清楚。
为研究覆盖厚度对CP处理后熏蒸效果的影响，本研究比较了CP熏蒸后覆盖0.06毫米（6S）和0.08毫米（8S）厚度薄膜的土壤温度、湿度、CP残留、微生物多样性和作物参数。
6S薄膜比8S薄膜降解更少，透明度、拉伸强度和断裂伸长率更高。与8S（F8S）相比，CP熏蒸（F6S）条件下6S地膜覆盖的土壤温度较低，土壤含水量较高。熏蒸第14天，F6S处理的CP含量比F8S处理高28.97%。F6S比F8S更有效地增加了有益微生物门和属，如、、，并减少了有害的和丛赤壳科。有益细菌OTU与地膜厚度（MT）、土壤温度（ST）和土壤含水量（SWC）显著相关。此外，F6S维持了根际微生物多样性平衡，抑制了病原菌（和）的积累，导致较高的幼苗成活率。上述变化进一步促进了药材生物量和皂苷的积累。总体而言，F6S处理提高了熏蒸效果以及药材的产量和品质，使其成为面临连作障碍的功能性块根作物土壤健康、品质和产量恢复的战略解决方案。
