基于细胞的ELISpot检测方法，用于检测和定量小鼠全器官单细胞悬液中抗原特异性抗体分泌细胞。
Cell-based ELISpot protocol to detect and quantify antigen-specific antibody-secreting cells in murine whole-organ single-cell suspensions.
作者信息
Brunner Christina, Menzel Shannon R, Rost Franziska, Côrte-Real Joana, Pracht Katharina
机构信息
Division of Molecular Immunology, Department of Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen, Nikolaus-Fiebiger Center, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany.
Division of Molecular Immunology, Department of Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen, Nikolaus-Fiebiger Center, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany.
出版信息
STAR Protoc. 2025 Jul 15;6(3):103797. doi: 10.1016/j.xpro.2025.103797.
Antibody-secreting cells (ASCs) are the hallmark of the adaptive immune response to vaccines or infections. Here, we present a protocol to quantify the number of ASCs using an enzyme-linked immunospot assay (ELISpot). We describe the preparation of cell samples, ELISpot plates, and their quantification. This assay has potential applications in vaccine studies and research on the adaptive immune system or ASC-associated diseases. For complete details on the use and execution of this protocol please, refer to Bierling et al..
抗体分泌细胞（ASC）是疫苗或感染适应性免疫反应的标志。在此，我们介绍一种使用酶联免疫斑点测定法（ELISpot）定量ASC数量的方案。我们描述了细胞样本、ELISpot板的制备及其定量方法。该测定法在疫苗研究以及适应性免疫系统或ASC相关疾病的研究中具有潜在应用。有关本方案使用和执行的完整详细信息，请参考比林等人的研究。
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