Wientzek M, Arthur G, Man R Y, Choy P C
J Lipid Res. 1985 Sep;26(9):1166-9.
We have developed a procedure for the determination of small amounts of lysophosphatidylcholine in cardiac tissue. Lysophosphatidylcholine from canine heart was separated from the major phospholipids by column chromatography, and then acetylated with labeled acetic anhydride. The acetylated lysophosphatidylcholine was isolated by thin-layer chromatography and the lysophosphatidylcholine content was calculated from the radioactivity associated with the acetylated product. Although the sensitivity of the assay depends on the specific radioactivity of the acetic anhydride used, as low as 0.5 nmol of lysophospholipid in tissue samples can be readily quantitated. The results obtained from the control and ischemic canine cardiac tissues by this assay compares favorably with those obtained by lipid-phosphorus assay. The sensitivity and specificity of the present procedure allows us and other investigators to assay for lysophosphatidylcholine content in very small (10 mg wet weight) tissue samples.
我们已经开发出一种用于测定心脏组织中少量溶血磷脂酰胆碱的方法。犬心脏中的溶血磷脂酰胆碱通过柱色谱法与主要磷脂分离，然后用标记的乙酸酐进行乙酰化。乙酰化的溶血磷脂酰胆碱通过薄层色谱法分离，溶血磷脂酰胆碱的含量根据与乙酰化产物相关的放射性来计算。尽管该测定法的灵敏度取决于所用乙酸酐的比放射性，但组织样品中低至0.5纳摩尔的溶血磷脂仍可轻松定量。通过该测定法从对照和缺血性犬心脏组织获得的结果与通过脂质磷测定法获得的结果相比具有优势。本方法的灵敏度和特异性使我们及其他研究人员能够测定非常小（10毫克湿重）的组织样品中的溶血磷脂酰胆碱含量。
