Ahmad F, Maier R, Mukherjee K D, Mangold H K
Lipids. 1987 Jun;22(6):413-6. doi: 10.1007/BF02537270.
Lipids containing hydroxy fatty acids or hydroxyacyl moieties are acetylated with [1-14C]acetic anhydride or [3H]acetic anhydride, and the content of hydroxy fatty acids or hydroxyacyl moieties is estimated from the specific radioactivity of the acetylated products with respect to that of radioactive standards, such as radioacetylated ricinoleic acid or triricinoleoylglycerol. Mixtures of triacylglycerols containing one, two and three hydroxyl groups per molecule are derivatized in a similar manner, and the resulting acetates are fractionated by thin layer chromatography according to the number of acetate groups per molecule. The relative proportion of each type of triacylglycerols in the mixture is estimated from the distribution of radioactivity in the various fractions. Applications of these techniques are demonstrated by the analysis of several seed lipids.
含有羟基脂肪酸或羟基酰基部分的脂质用[1-¹⁴C]乙酸酐或[³H]乙酸酐进行乙酰化，然后根据乙酰化产物相对于放射性标准品（如放射性乙酰化蓖麻油酸或三蓖麻酰甘油）的比放射性来估算羟基脂肪酸或羟基酰基部分的含量。每分子含有一个、两个和三个羟基的三酰甘油混合物以类似方式进行衍生化，所得乙酸酯根据每分子的乙酸酯基团数量通过薄层色谱法进行分离。混合物中每种类型三酰甘油的相对比例根据放射性在各个馏分中的分布来估算。通过对几种种子脂质的分析展示了这些技术的应用。
