Vanluchene E, Vandekerckhove D, Jonckheere J, De Leenheer A
J Chromatogr. 1983 Nov 25;279:573-80. doi: 10.1016/s0021-9673(01)93659-3.
Steroid profiling by capillary gas chromatography has been applied to the analysis of free and conjugated steroids in serum, ovarian follicles, lutein cyst and peritoneal fluids. Sep-Pak C18 octadecylsilica was used for extraction, whereas the separation of free and different conjugates was accomplished by successive hydrolysis and liquid extraction steps.