Ausman L M, Harwood J P, King N W, Sehgal P K, Nicolosi R J, Hegsted D M, Liener I E, Donatucci D, Tarcza J
J Nutr. 1985 Dec;115(12):1691-701. doi: 10.1093/jn/115.12.1691.
Twenty-seven 2- to 4-yr-old cebus monkeys (Cebus albifrons) were fed from infancy purified diets containing lactalbumin, soy isolate, casein or soy concentrate as the sole protein source. Hematologic and clinical chemistry values were similar for all groups. Head and tail portions of each pancreas were surgically removed for histopathologic evaluation and determination of protein, RNA and DNA content, and for trypsin and chymotrypsin activity. Hematoxylin and eosin-stained sections from 26 of 27 monkeys showed normal pancreatic tissue with occasional acinar vacuolation in all diet groups. The remaining animal, one of only two fed soy concentrate, had diffuse interstitial fibrosis of the pancreas associated with mild to moderate atrophy of acinar tissue. Biochemical analyses of the pancreatic biopsies indicated no group differences among animals fed lactalbumin, soy isolate or casein. One of two monkeys in the soy concentrate group showed decreased pancreatic protein, RNA and trypsin concentrations; this was probably due to the fibrosis in this animal. No evidence of pancreatic hypertrophy or hyperplasia, as measured by RNA/DNA and protein/DNA ratios, respectively, was seen in any diet group.
27只2至4岁的白额卷尾猴（Cebus albifrons）从幼年起就被喂食以乳白蛋白、大豆分离蛋白、酪蛋白或大豆浓缩蛋白作为唯一蛋白质来源的纯化日粮。所有组的血液学和临床化学值相似。通过手术切除每个胰腺的头部和尾部，用于组织病理学评估以及蛋白质、RNA和DNA含量的测定，还有胰蛋白酶和糜蛋白酶活性的测定。27只猴子中有26只的苏木精-伊红染色切片显示所有日粮组的胰腺组织正常，偶尔有腺泡空泡化。其余的一只猴子（仅有的两只喂食大豆浓缩蛋白的猴子之一）胰腺有弥漫性间质纤维化，伴有腺泡组织轻度至中度萎缩。胰腺活检的生化分析表明，喂食乳白蛋白、大豆分离蛋白或酪蛋白的动物之间没有组间差异。大豆浓缩蛋白组的两只猴子中有一只胰腺蛋白质、RNA和胰蛋白酶浓度降低；这可能是由于该动物存在纤维化。在任何日粮组中，分别通过RNA/DNA和蛋白质/DNA比率测量，均未发现胰腺肥大或增生的迹象。
