Sayarcan Döne, Biçer Ahmet, Şahinduran Şima, Uluğ Bülent, Cicek Ahmet
Opticianry Programme, Gölhisar Vocational School of Health Services, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye.
Department of Physics, Faculty of Arts and Science, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye.
Vet Med Sci. 2025 Jul;11(4):e70490. doi: 10.1002/vms3.70490.
A portable, lightweight, and compact microscope was developed for rapid on-site identification of Cryptosporidium spp. oocysts, a major cause of diarrhoea in neonatal ruminants. Cryptosporidiosis is an infectious parasitic disease that causes diarrhoea, and rapid diagnosis plays a critical role in initiating timely treatment. A method for diagnosing cryptosporidiosis involves detecting oocysts excreted in faeces using a light microscope with an appropriate staining method. However, transporting samples to a laboratory often takes time, during which the animal's health may deteriorate. The developed portable microscope provides up to 1000x magnification, enabling the diagnosis of cryptosporidiosis using the carbol fuchsin staining method. It is compatible with almost any smartphone and does not require additional digital or optical magnification. Through an integrated mobile telemedicine application, veterinarians can instantly consult specialists by transmitting patient information and images. Calibration tests have demonstrated that the system can generate high-resolution images of oocysts. This innovative microscope is a crucial tool for veterinarians working in the field, allowing for rapid detection of Cryptosporidium spp. oocysts, reducing neonatal calf mortality, and preventing the spread of infectious diseases.
一种便携式、轻便且紧凑的显微镜被开发出来，用于快速现场鉴定隐孢子虫属卵囊，这是新生反刍动物腹泻的主要原因。隐孢子虫病是一种导致腹泻的传染性寄生虫病，快速诊断在及时开展治疗方面起着关键作用。一种诊断隐孢子虫病的方法是使用配备适当染色方法的光学显微镜检测粪便中排出的卵囊。然而，将样本运送到实验室往往需要时间，在此期间动物的健康状况可能会恶化。所开发的便携式显微镜提供高达1000倍的放大倍数，能够使用石炭酸品红染色法诊断隐孢子虫病。它几乎与任何智能手机兼容，并且不需要额外的数字或光学放大。通过集成的移动远程医疗应用程序，兽医可以通过传输患者信息和图像立即咨询专家。校准测试表明，该系统可以生成卵囊的高分辨率图像。这种创新型显微镜是在野外工作的兽医的重要工具，能够快速检测隐孢子虫属卵囊，降低新生犊牛死亡率，并防止传染病传播。
