Zhong Shengqiang, Zou Hongwei, Hou Chao, Yang Fan, Zeng Kaibin, Liu Yuhan, Huang Yongsheng, Jiang Xiantao
School of Science, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.
Biomed Opt Express. 2025 May 6;16(6):2275-2282. doi: 10.1364/BOE.560024. eCollection 2025 Jun 1.
This study introduces a vortex beam speckle imaging system for quantitative phase imaging (QPI) with high lateral resolution. By introducing vortex beams for non-diffracting speckle field regulation, the speckle size can be significantly reduced from 116.32 μm to 11.07 μm. With these advantages, the proposed imaging system has shown 1.52 folds of lateral resolution improvement compared to a traditional coherent imaging system. Furthermore, the intensity signal-to-noise ratio of the imaging system has also been improved from 13.26 dB to 30.62 dB. Transport-of-intensity equation (TIE) phase retrieval algorithms were applied to standard quantitative phase targets, and red blood cell samples were used to demonstrate the system's precise phase retrieval capability, indicating its potential applications for label-free, non-invasive biomedical imaging.
本研究介绍了一种用于高横向分辨率定量相位成像（QPI）的涡旋光束散斑成像系统。通过引入涡旋光束来调控非衍射散斑场，散斑尺寸可从116.32 μm显著减小至11.07 μm。凭借这些优势，与传统相干成像系统相比，所提出的成像系统横向分辨率提高了1.52倍。此外，成像系统的强度信噪比也从13.26 dB提高到了30.62 dB。将强度传输方程（TIE）相位检索算法应用于标准定量相位目标，并使用红细胞样本证明了该系统精确的相位检索能力，表明其在无标记、非侵入性生物医学成像方面的潜在应用。
