Li Jilang, Qiu Haixin, Li Siyuan, Han Shan, He Yuming, He Jia, Gao Xiang, Li Jingjing, Feng Jianfang, Yang Shilin, Yuan Renyikun, Gao Hongwei
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China.
Engineering Research Center of Innovative Drugs for Traditional Chinese Medicine and Zhuang & Yao Medicine, Ministry of Education, Nanning, 530000, China.
J Tradit Complement Med. 2024 Apr 16;15(4):356-368. doi: 10.1016/j.jtcme.2024.04.001. eCollection 2025 Jul.
Pulsatilla saponin (Ps) was isolated from Pulsatilla chinensis (Bunge) Regel, a traditional Chinese medicine, that has anti-proliferation, anti-inflammation, anti-tumor and immunomodulation activities. However, the anti-psoriasis activity of Ps and its underlying mechanisms have not been fully elucidated. This study aims to investigate the effect and potential mechanisms of Ps on psoriasis.
Ps underwent quality control through HPLC and NMR analysis. Wound healing assay, MTT, clone assay, and EdU staining were used to detect HaCaT cells proliferation. Western blot and immunofluorescence were used to assess the expression of proteins. The th17 cells population was analyzed by flow cytometry. The levels of cytokines in the mice skin tissues were measured by RT-qPCR and ELISA.
In vitro, Ps has an inhibition effect on the proliferation of M5-induced HaCaT cells. Ps inhibited proliferation by regulating NF-κB and JAK1/STAT3 pathways. Additionally, Ps decreased TNF-α, IL-1β, and IL-6 mRNA levels in M5-induced HaCaT cells. In vivo, Ps improved the pathological damage of Imiquimod (IMQ)-induced psoriasis BALB/c mice skin and reduced the Ki67 level in mice skin tissue. Further results showed that Ps decreased Th17 cells differentiation and IL-22, IL-17A, IL-6, IFN-γ, TNF-α, and IL-1β secretion. Ps could ameliorate the psoriatic symptoms, decrease M5-induced HaCaT cell proliferation, and decrease the differentiation of Th17 cells in IMQ-induced psoriasis mice. Ps suppressed the release of inflammation cytokines by regulating NF-κB and JAK1/STAT3 pathways. Those results indicate that Ps has promising therapeutic potential for psoriasis treatment.
白头翁皂苷（Ps）是从传统中药白头翁中分离得到的，具有抗增殖、抗炎、抗肿瘤及免疫调节活性。然而，Ps的抗银屑病活性及其潜在机制尚未完全阐明。本研究旨在探讨Ps对银屑病的作用及潜在机制。
通过高效液相色谱（HPLC）和核磁共振（NMR）分析对Ps进行质量控制。采用伤口愈合试验、MTT法、克隆试验和EdU染色检测HaCaT细胞增殖。采用蛋白质免疫印迹法和免疫荧光法评估蛋白质表达。通过流式细胞术分析Th17细胞群体。采用逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）检测小鼠皮肤组织中细胞因子水平。
在体外，Ps对M5诱导的HaCaT细胞增殖具有抑制作用。Ps通过调节核因子κB（NF-κB）和Janus激酶1/信号转导与转录激活因子3（JAK1/STAT3）信号通路抑制增殖。此外，Ps降低了M5诱导的HaCaT细胞中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的信使核糖核酸（mRNA）水平。在体内，Ps改善了咪喹莫特（IMQ）诱导的银屑病BALB/c小鼠皮肤的病理损伤，并降低了小鼠皮肤组织中Ki67水平。进一步结果表明，Ps减少了Th17细胞分化以及白细胞介素-22（IL-22）、白细胞介素-17A（IL-17A）、IL-6、干扰素-γ（IFN-γ）、TNF-α和IL-1β的分泌。Ps可改善银屑病症状，减少M5诱导的HaCaT细胞增殖，并减少IMQ诱导的银屑病小鼠中Th17细胞的分化。Ps通过调节NF-κB和JAK1/STAT3信号通路抑制炎症细胞因子的释放。这些结果表明，Ps在银屑病治疗方面具有广阔的治疗潜力。
