Tuzlakoğlu Öztürk Merve
Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Gebze, Kocaeli, Turkiye.
Turk J Biol. 2025 Mar 17;49(3):273-279. doi: 10.55730/1300-0152.2744. eCollection 2025.
BACKGROUND/AIM: CCDC43 (coiled-coil domain containing protein 43) is a eukaryotic protein that contains alpha-helical domains in its structure, consistent with the family to which it belongs. It is predominantly located in the cytosol of the cell. CCDC43 protein has been shown to play a role in cell proliferation, invasion, metastasis, and epithelial-mesenchymal transition in gastric and colorectal cancer types. The fundamental function and cellular interaction network of this protein, which is known to have varying expression levels in various cancer types, has not yet been fully elucidated. Here, we conducted a proximity-dependent biotin identification (BioID2) screening approach for CCDC43 to uncover proximity interactors.
Using mass spectrometry of streptavidin pull-down of biotinylated proteins with filtering approaches, we identified candidate protein interactors for CCDC43.
We suggest an association between CCDC43 and RNA-binding proteins based on all our biological replicates. The strongest candidate for the interactome is YBX1, a highly conserved cold shock domain and nucleic acid-binding protein that has multiple essential functions in the cell.
These findings suggest that CCDC43 may play a role in critical pathways within the cell.
背景/目的：CCDC43（含卷曲螺旋结构域蛋白43）是一种真核蛋白，其结构中含有α-螺旋结构域，与其所属家族一致。它主要位于细胞的胞质溶胶中。CCDC43蛋白已被证明在胃癌和结直肠癌类型的细胞增殖、侵袭、转移及上皮-间质转化中发挥作用。这种在各种癌症类型中表达水平各异的蛋白的基本功能和细胞相互作用网络尚未完全阐明。在此，我们对CCDC43进行了邻近依赖性生物素识别（BioID2）筛选，以发现邻近相互作用蛋白。
通过对生物素化蛋白进行链霉亲和素下拉并结合过滤方法的质谱分析，我们鉴定了CCDC43的候选蛋白相互作用体。
基于所有生物学重复实验，我们提示CCDC43与RNA结合蛋白之间存在关联。相互作用组中最有力的候选蛋白是YBX1，它是一种高度保守的冷休克结构域和核酸结合蛋白，在细胞中具有多种重要功能。
这些发现提示CCDC43可能在细胞内的关键通路中发挥作用。
