Gou Xiaoling, Chen Yuqing, Zong Yi, Huang Xuemei, Shen Yihong, Wang Lijie, Liu Yifan, He Yuchi, Jia Jialong, Zhang Xiyu, Peng Sihan, Zhou Xianhua, Liu Ya, Zhang Jing, Fan Gang
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Ethnic Medicine and Meishan Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.
Department of Endocrinology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P.R. China.
iScience. 2025 Jun 9;28(7):112856. doi: 10.1016/j.isci.2025.112856. eCollection 2025 Jul 18.
Diabetes related macroangiopathy (DMA) is a major complication of type 2 diabetes (T2D), impacting both morbidity and mortality. This study characterized the gut bacteriome and mycobiome in 179 adults, including 58 with DMA, 71 with T2D, and 50 healthy controls. The gut microbiome of DMA subjects exhibited reduced alpha diversity, and a distinct microbial composition compared with healthy control. Two bacterial families, six bacterial genera, and four bacterial species exhibited significant differences between DMA and T2D subjects. Additionally, in the mycobiome group, Xylariales was significantly decreased in DMA subjects compared with T2D subjects. Disruptions in transkingdom interactions between gut bacteria and fungi supported microbiota dysbiosis in DMA. A diagnostic model combining bacterial and fungal markers achieved an AUC of 94.20%. This work deepens our understanding of the microbial landscape associated with macroangiopathy in diabetes and highlights potential microbial targets for diagnostics and therapeutic intervention.
糖尿病相关大血管病变（DMA）是2型糖尿病（T2D）的主要并发症，影响发病率和死亡率。本研究对179名成年人的肠道细菌群落和真菌群落进行了特征分析，其中包括58名患有DMA的患者、71名患有T2D的患者和50名健康对照者。与健康对照相比，DMA患者的肠道微生物群α多样性降低，且微生物组成不同。两个细菌科、六个细菌属和四个细菌种在DMA患者和T2D患者之间表现出显著差异。此外，在真菌群落组中，与T2D患者相比，DMA患者的炭角菌目显著减少。肠道细菌和真菌之间跨界相互作用的破坏支持了DMA中的微生物群失调。结合细菌和真菌标志物的诊断模型的曲线下面积（AUC）达到94.20%。这项工作加深了我们对糖尿病大血管病变相关微生物景观的理解，并突出了诊断和治疗干预的潜在微生物靶点。
