Chatenoud Holly, Łaniewski Paweł, Mahnert Nichole D, Herbst-Kralovetz Melissa M
Department of Basic Medical Sciences, College of Medicine - Phoenix, University of Arizona, Phoenix, AZ 85004, USA.
Department of Life Sciences, University of Bath, Bath BA2 7AY, UK.
iScience. 2025 Jun 18;28(7):112938. doi: 10.1016/j.isci.2025.112938. eCollection 2025 Jul 18.
In this pilot study, we investigated three minimally to noninvasive biospecimens: cervicovaginal lavages (CVLs), vaginal swabs, and urine, from 18 premenopausal women using untargeted metabolomics to inform future gynecologic research. Metabolic profiles were compared between specimen types and between participants grouped based on vaginal microbiome composition and body mass index (BMI). We identified metabolites altered in the groups in all sample types; however, different metabolites were uniquely detected in each biospecimen. Within CVLs, key amino acid signatures (i.e., polyamines) were identified in women with dysbiosis. While polyamines were also detected in vaginal swabs, this sample type exhibited more lipid signatures. In urine, we specifically detected estrogenic steroids, endocrine-disrupting compounds, and certain drugs. Overall, each urogenital biospecimen type exhibits unique metabolic profiles and, based on metabolic alteration in the context of vaginal microbiome and BMI, can be valuable tools in answering questions related to gynecologic health.
在这项初步研究中，我们使用非靶向代谢组学方法，对18名绝经前女性的三种微创至无创生物标本进行了研究，这三种标本分别是宫颈阴道灌洗液（CVL）、阴道拭子和尿液，旨在为未来的妇科研究提供信息。我们比较了不同标本类型之间以及根据阴道微生物群组成和体重指数（BMI）分组的参与者之间的代谢谱。我们在所有样本类型的组中都鉴定出了代谢物的变化；然而，每种生物标本中都独特地检测到了不同的代谢物。在CVL中，我们在患有菌群失调的女性中鉴定出了关键的氨基酸特征（即多胺）。虽然在阴道拭子中也检测到了多胺，但这种样本类型表现出更多的脂质特征。在尿液中，我们特别检测到了雌激素类固醇、内分泌干扰化合物和某些药物。总体而言，每种泌尿生殖系统生物标本类型都呈现出独特的代谢谱，并且基于阴道微生物群和BMI背景下的代谢变化，它们可以成为回答与妇科健康相关问题的有价值工具。
