Abouelenin Mai A H, Saleh Amany A, Elabd Naglaa S, Elbahr Osama, Abdelaziz Reham Ahmed, Elhelbawy Mohammed G, Kasemy Zeinab A, Hussein Ahmed Abdelkhalek, Abbas Mona A
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, 32511, Egypt.
Medical Surgical Nursing Department, College of Nursing, Taibah University, KSA, Madina, Saudi Arabia.
Biochem Biophys Rep. 2025 Jun 27;43:102117. doi: 10.1016/j.bbrep.2025.102117. eCollection 2025 Sep.
Gastric cancer ranks fourth in both incidence and mortality worldwide. Several genes influence its genesis and progress.
The expression of MBNL3, PXN genes, and lncRNA PXN-AS1-L was evaluated in gastric tissue samples from 75 gastric cancer patients and 75 controls by RT-PCR.
MBNL3, PXN, and lncRNA PXN-AS1-L expressions were considerably raised among cancerous gastric tissue than in nearby normal tissue and normal tissue from controls (p < 0.001). LncRNA PXN-AS1-L and MBNL3 had the highest sensitivity (92.00 % and 90.67 %, respectively), with PXN sensitivity of 81.33 % for differentiation of the cancer tissue from adjacent normal tissue, while PXN and lncRNA PXN-AS1-L had the highest sensitivity (98.67 % and 92.00 %, respectively), with 89.33 % for MBNL3 as discriminators between gastric cancer tissue and normal tissue from controls. Between metastatic and non-metastatic patients, MBNL3 and PXN were the highest differentiating markers (sensitivity = 87.50 % and 83.33 %, respectively). MBNL3, PXN, and lncRNA PXN-AS1-L were reliable predictors of gastric cancer (p = 0.004, p = 0.009, and p = 0.001, respectively), but only MBNL3 and lncRNA PXN-AS1-L were significant predictors for mortality (p = 0.026 and p = 0.043, respectively). The elevated expressions were linked to lower overall and progression-free survival rates.
MBNL3, PXN, and lncRNA PXN-AS1-L expression levels can diagnose and predict gastric cancer and discriminate between metastatic and non-metastatic patients. The expression level of MBNL3 and lncRNA PXN-AS1-L can predict gastric cancer mortality. Higher expression of MBNL3 and PXN was linked to aggressive gastric cancer and low overall and progression-free survival rates.
胃癌的发病率和死亡率在全球范围内均位居第四。多种基因影响其发生和发展。
采用逆转录聚合酶链反应（RT-PCR）检测75例胃癌患者和75例对照者胃组织样本中MBNL3、PXN基因及lncRNA PXN-AS1-L的表达。
与癌旁正常组织及对照者的正常组织相比，胃癌组织中MBNL3、PXN及lncRNA PXN-AS1-L的表达显著升高（p < 0.001）。lncRNA PXN-AS1-L和MBNL3的灵敏度最高（分别为92.00%和90.67%），PXN区分癌组织与相邻正常组织的灵敏度为81.33%，而PXN和lncRNA PXN-AS1-L的灵敏度最高（分别为98.67%和92.00%），MBNL3区分胃癌组织与对照者正常组织的灵敏度为89.33%。在有转移和无转移患者之间，MBNL3和PXN是最具鉴别力的标志物（灵敏度分别为87.50%和83.33%）。MBNL3、PXN及lncRNA PXN-AS1-L是胃癌的可靠预测指标（p分别为0.004、0.009和0.001），但只有MBNL3和lncRNA PXN-AS1-L是死亡率的显著预测指标（p分别为0.026和0.043）。表达升高与较低的总生存率和无进展生存率相关。
MBNL3、PXN及lncRNA PXN-AS1-L的表达水平可用于诊断和预测胃癌，并区分有转移和无转移患者。MBNL³和lncRNA PXN-AS1-L的表达水平可预测胃癌死亡率。MBNL3和PXN的高表达与侵袭性胃癌及较低的总生存率和无进展生存率相关。
