Aldı Feza İrem, Tabakçıoglu Kıymet, Polat Erdal, Şakru Nermin
Trakya University Faculty of Medicine, Department of Medical Microbiology, Edirne, Türkiye.
Trakya University Faculty of Medicine Department of Medical Biology, Edirne, Türkiye.
Turkiye Parazitol Derg. 2025 Jul 18;49(2):82-87. doi: 10.4274/tpd.galenos.2025.36854.
causes echinococcosis when its larvae settle in various organs, especially the liver and lung, of humans and herbivorous animals such as sheep and cattle. Echinococcosis are endemic in Mediterranean countries including Türkiye, the Middle East and South Africa. Echinococcosis treatments are generally surgical excision or benzimidazoles. Maggot debridement therapy is a wound treatment with larvae. While the larvae consume the old-damaged tissues and bacteria in the wound site, the maggots secrete an antimicrobial fluid. In this study, we aimed to determine the effect of larval secretions on protoscoleces .
Various DNA damage markers were used to analyze the therapeutic potential of the larval secretions. For this purpose, protoscoleces were cultured and treated with different concentrations of larval secretions. Comet test was performed to determine DNA damage. Expression of and genes was analyzed by quantitative real time polymerase chain reaction.
The viability of the control group was 94% and the viability of the protoscoleces treated with larval secretions was 73%. Comet test showed that larval secretions caused DNA damage in protoscoleces. According to quantitative real time polymerase chain reaction results; 1:1 larval secretions increased and gene expression 3.2-fold and gene expression 2.2-fold compared to control groups (p<0.05).
These data showed that in vitro larval secretion induced DNA damage in protoscoleces and increased the expression of and genes. However, further and studies are needed.
当幼虫定居于人类以及绵羊和牛等食草动物的各种器官，尤其是肝脏和肺部时，会引发棘球蚴病。棘球蚴病在地中海国家（包括土耳其、中东和南非）呈地方性流行。棘球蚴病的治疗通常为手术切除或使用苯并咪唑类药物。蛆虫清创疗法是一种使用幼虫的伤口治疗方法。当幼虫消耗伤口部位的陈旧受损组织和细菌时，蛆虫会分泌一种抗菌液。在本研究中，我们旨在确定幼虫分泌物对原头节的影响。
使用各种DNA损伤标记物来分析幼虫分泌物的治疗潜力。为此，培养原头节并用不同浓度的幼虫分泌物进行处理。进行彗星试验以确定DNA损伤。通过定量实时聚合酶链反应分析基因和基因的表达。
对照组的存活率为94%，用幼虫分泌物处理的原头节的存活率为73%。彗星试验表明幼虫分泌物会导致原头节的DNA损伤。根据定量实时聚合酶链反应结果；与对照组相比，1:1的幼虫分泌物使基因和基因表达增加了3.2倍，基因表达增加了2.2倍（p<0.05）。
这些数据表明，体外幼虫分泌物会诱导原头节的DNA损伤，并增加基因和基因的表达。然而，还需要进一步的和研究。
