Mohanty Barsha, Mohan Masmarika, Nanjappa Dechamma Pandyanda, Shenoy Rathika D, Hosmane Giridhar B, Chakraborty Gunimala, Chakraborty Anirban
Department of Molecular Genetics and Cancer, NITTE (Deemed to Be University), Nitte University Centre for Science Education and Research (NUCSER), Mangaluru, Karnataka, India.
Department of Pediatrics, NITTE (Deemed to be University), K.S. Hegde Medical Academy (KSHEMA), Mangaluru, Karnataka, India.
WIREs Mech Dis. 2025 Jul-Aug;17(4):e70002. doi: 10.1002/wsbm.70002.
Molecular research has gradually revealed the biological significance of genetically encoded information and how this information is transmitted and utilized in a cell. The scientific advances of the last few decades have brought about paradigm shifts in the strategies traditionally used to decipher biological information. From unidirectional approaches, we now have multidirectional model-system-based integrated OMICs that aim to describe the pathophysiology of diseases through a combination of genetic, transcriptomic, proteomic, and metabolomic data. Compared to other vertebrate models, zebrafish have a wealth of advantages that make them a powerful tool with a wide range of applications in biomedical research. The high degree of genetic conservation with humans, coupled with the availability of various gene manipulation techniques, has made zebrafish an immensely popular multi-utility genetic toolbox. This review describes the advances in the field of zebrafish-based biomedical research with a focus on its applications in disease modeling, functional omics, toxicology, and pharmacology. This article is categorized under: Cancer > Genetics/Genomics/Epigenetics Infectious Diseases > Molecular and Cellular Physiology Congenital Diseases > Molecular and Cellular Physiology.
分子研究逐渐揭示了遗传编码信息的生物学意义，以及这些信息在细胞中如何传递和利用。过去几十年的科学进展使传统上用于解读生物信息的策略发生了范式转变。从单向方法，我们现在有了基于多向模型系统的综合组学，旨在通过整合遗传、转录组、蛋白质组和代谢组数据来描述疾病的病理生理学。与其他脊椎动物模型相比，斑马鱼具有诸多优势，使其成为生物医学研究中具有广泛应用的强大工具。与人类高度的遗传保守性，再加上各种基因操作技术的可用性，使斑马鱼成为一个极其受欢迎的多功能遗传工具箱。本综述描述了基于斑马鱼的生物医学研究领域的进展，重点关注其在疾病建模、功能组学、毒理学和药理学中的应用。本文分类如下：癌症>遗传学/基因组学/表观遗传学；传染病>分子和细胞生理学；先天性疾病>分子和细胞生理学。
