Yazici Zafer, Baskin Huseyin, Gozel Seda, Kurucay Hanne Nur, Tamer Cuneyt, Kadi Hamza, Ozan Emre, Muftuoglu Bahadir, Bayrakal Vahide, Albayrak Harun, Okur-Gumusova Semra, Elhag Ahmed Eisa
Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Türkiye.
Department of Medical Microbiology, Faculty of Medicine, Dokuz Eylul University, Izmir, Türkiye.
PLoS One. 2025 Jul 18;20(7):e0328556. doi: 10.1371/journal.pone.0328556. eCollection 2025.
Human adenoviruses (HAdV) are significant etiological agents of infections affecting the respiratory, gastrointestinal, urinary and ocular systems, particularly in adults, infants, and immunocompromised individuals. This study presents the molecular identification of a local HAdV strain for the first time from the stool of a healthy infant in Türkiye, isolated in 2003 and stored for two decades in liquid nitrogen. Molecular characterization of this strain was performed, identifying it as HAdV-C6. Phylogenetic analysis revealed high nucleotide identity (97%) with global strains from Russia, China, Japan, and the USA. A serum neutralization test was conducted to determine the current circulation of this strain, indicating a 9.5% seropositivity rate in archival serum samples collected for the West Nile virus surveillance project. This study provides insights into the persistence and molecular epidemiology of HAdV strains circulating in Türkiye, highlighting the need for continuous surveillance and whole-genome sequencing to assess potential recombination events and genetic variations.
人腺病毒（HAdV）是影响呼吸道、胃肠道、泌尿系统和眼部系统感染的重要病原体，尤其是在成人、婴儿和免疫功能低下的个体中。本研究首次从一名健康婴儿的粪便中对一株本地HAdV毒株进行了分子鉴定，该毒株于2003年分离，在液氮中保存了二十年。对该毒株进行了分子特征分析，确定其为HAdV-C6。系统发育分析显示，它与来自俄罗斯、中国、日本和美国的全球毒株具有较高的核苷酸同一性（97%）。进行了血清中和试验以确定该毒株的当前流行情况，结果表明在为西尼罗河病毒监测项目收集的存档血清样本中，血清阳性率为9.5%。本研究为土耳其境内传播的HAdV毒株的持续性和分子流行病学提供了见解，强调了持续监测和全基因组测序以评估潜在重组事件和基因变异的必要性。
