Zhang Xinmeng, Jiao Yue, Yang Taikang, Xu Zhourui, Shao Yonghong, Hou Jianxun, Xu Gaixia
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China.
Shenzhen Testing Center of Medical Devices, Shenzhen Institute for Drug Control, Shenzhen, 518057, China.
Mikrochim Acta. 2025 Jul 19;192(8):505. doi: 10.1007/s00604-025-07292-w.
The synergy between electroosmotic flow (EOF) and surface plasmon resonance (SPR) offers a transformative approach to overcome diffusion-limited biosensing. However, existing voltage-driven EOF systems face critical trade-offs between high-voltage requirements and biocompatibility. Here, we present a self-powered electroosmosis-enhanced SPR immunosensor that integrates a triboelectric nanogenerator (TENG) with SPR imaging (SPRi) to achieve ultra-sensitive detection of HER2-positive cancer cells. By leveraging TENG-driven asymmetric alternating electric fields, the developed platform generates electroosmotic vortices that accelerate cellular transport velocity and enhance antigen-antibody binding efficiency, achieving a detection limit of 3 × 10 cells/mL-an order of magnitude improvement over label-free SPR methods. Remarkably, the system captures HER2-positive cells within 45 s at a high throughput of cell, the detection speed increased by 20-fold. This performance, combined with label-free operation and biocompatibility, establishes a scalable framework for liquid biopsy applications.
电渗流（EOF）与表面等离子体共振（SPR）之间的协同作用为克服扩散限制的生物传感提供了一种变革性方法。然而，现有的电压驱动电渗流系统在高电压要求和生物相容性之间面临关键权衡。在此，我们展示了一种自供电电渗增强SPR免疫传感器，该传感器将摩擦纳米发电机（TENG）与SPR成像（SPRi）集成，以实现对HER2阳性癌细胞的超灵敏检测。通过利用TENG驱动的不对称交变电场，所开发的平台产生电渗涡旋，加速细胞传输速度并提高抗原-抗体结合效率，实现了3×10个细胞/毫升的检测限——比无标记SPR方法提高了一个数量级。值得注意的是，该系统在45秒内以高通量捕获HER2阳性细胞，检测速度提高了20倍。这种性能，结合无标记操作和生物相容性，为液体活检应用建立了一个可扩展的框架。
