Yoshikawa M, Arashidani K, Kodama Y
Sangyo Igaku. 1985 Mar;27(2):83-9. doi: 10.1539/joh1959.27.83.
A simple analytical method for urinary phenol by high performance liquid chromatography was tested. This procedure consists of the following three steps: hydrolysis of urine with hydrochloric acid, extraction of phenols with isopropylether, separation and determination of phenol by high performance liquid chromatography, under the following operating conditions: column, LiChrosorb RP 18 (250 mm X 4 mm, I.D.); mobile phase, acetonitrile-water (3: 7, v/v) with a flow rate of 0.8 ml/min; column temperature, 40 degrees C; detection, UV monitor at 270 nm. The detection limit (signal-to-noise ratio = 2) was 1 ng. The analytical results by this method were compared with those obtained by another method used for gas chromatography. The analytical results obtained by the two methods were in good agreement (r = 0.998) for 30 normal urine samples tested. The geometrical average value for phenol in normal urine samples (n = 30) was 8.2 micrograms/ml. Therefore this method was proved to be useful for biological monitoring of benzene exposure.
对一种通过高效液相色谱法检测尿酚的简单分析方法进行了测试。该程序包括以下三个步骤：用盐酸水解尿液，用异丙醚萃取酚类，在以下操作条件下通过高效液相色谱法分离和测定酚类：色谱柱，LiChrosorb RP 18（250 mm×4 mm，内径）；流动相，乙腈 - 水（3:7，v/v），流速为0.8 ml/min；柱温，40℃；检测，在270 nm处用紫外监测器。检测限（信噪比 = 2）为1 ng。将该方法的分析结果与另一种用于气相色谱法的方法所获得的结果进行了比较。对于所测试的30份正常尿液样本，两种方法获得的分析结果具有良好的一致性（r = 0.998）。正常尿液样本（n = 30）中酚的几何平均值为8.2微克/毫升。因此，该方法被证明可用于苯暴露的生物监测。
