Srikar Anagoni, Nagaraja Mudhigeti, Mohan Alladi, Sireesha Kodavala, Verma Anju, Kalawat Usha
Department of Microbiology, Father Colombo Institute of Medical Sciences, Warangal, Telangana 506001, India.
Department of Microbiology, Sri Balaji Medical College Hospital and Research Institute, Renigunta, Tirupati, Andhra Pradesh 517520, India.
Diagn Microbiol Infect Dis. 2025 Nov;113(3):116996. doi: 10.1016/j.diagmicrobio.2025.116996. Epub 2025 Jul 8.
Healthcare workers (HCWs) are a well-known risk group for coronavirus infection with increased working hours in a potentially infectious environment and immunity levels to be monitored.
1001 HCWs were tested for both IgG and Neutralizing antibodies. IgG avidity was tested in 100 HCWs using 3 M, 5 M and 7 M urea concentration. One-year follow-up testing for both IgG and Neutralizing antibodies was performed in 151 HCWs.
COVID-19 IgG antibody levels were high among 299 (94.62 %) HCWs with a history of COVID-19 infection (p <0.0001) compared to 479 (69.92 %) HCWs who were not infected with COVID-19 during the first and second waves. A total of 899 (89.81 %) HCWs had more than 50 % neutralizing antibodies, whereas the remaining 102 (10.19 %) HCWs had less than 50 % neutralizing antibodies. The avidity index was maintained at approximately 40 % (grey zone). In our study, 5.1 folds the IgG levels increased after 1 year compared to the initial results, with a 6.3 folds increase in the high-risk group compared to 3.6 folds increase in the low-risk group.
Healthcare workers with a higher infection rate and two-fold higher IgG levels in HCWs involved in COVID-19 duty and their persistence for a longer time than in other groups signify sustained antibody responses of IgG antibodies, which play a crucial role in reducing the severity of disease in HCWs involved in COVID-19 patient care and may provide protection against emerging SARS-CoV-2 variants.
医护人员是冠状病毒感染的一个众所周知的风险群体，他们在潜在感染环境中的工作时间增加，且免疫水平有待监测。
对1001名医护人员进行了IgG和中和抗体检测。使用3M、5M和7M尿素浓度对100名医护人员进行了IgG亲和力检测。对151名医护人员进行了IgG和中和抗体的一年随访检测。
与在第一波和第二波期间未感染新冠病毒的479名医护人员（69.92%）相比，有新冠病毒感染史的299名医护人员（94.62%）的新冠病毒IgG抗体水平较高（p<0.0001）。共有899名医护人员（89.81%）的中和抗体超过50%，而其余102名医护人员（10.19%）的中和抗体低于50%。亲和力指数维持在约40%（灰色区域）。在我们的研究中，与初始结果相比，1年后IgG水平增加了5.1倍，高危组增加了6.3倍，而低危组增加了3.6倍。
参与新冠疫情防控工作的医护人员感染率较高，IgG水平比其他群体高出两倍，且持续时间更长，这表明IgG抗体具有持续的抗体反应，在降低参与新冠患者护理的医护人员疾病严重程度方面发挥着关键作用，并可能提供针对新出现的新冠病毒变异株的保护。
