Shinohara Koh, Tsuda Yusuke, Tsuchido Yasuhiro, Yamamoto Masaki, Matsumura Yasufumi, Nagao Miki
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
J Infect. 2025 Aug;91(2):106552. doi: 10.1016/j.jinf.2025.106552. Epub 2025 Jul 18.
We conducted a multicenter retrospective study to characterize the clinical features of listeriosis and the genomic characteristics of causative Listeria monocytogenes isolates across Japan.
In this retrospective observational cohort study, we enrolled all microbiologically confirmed listeriosis cases between Jan 1, 2011, and Dec 31, 2021. We collected the causative isolates and performed whole genome sequencing. The isolates were compared using Multi-locus Sequence Typing (MLST), core-genome MLST, and single-nucleotide polymorphism (SNP)-based phylogeny.
A total of 195 listeriosis cases from 22 hospitals were enrolled, including 134 cases of bacteremia, 43 cases of neurolisteriosis, 11 cases of perinatal infection, and 7 cases of other infections. Of the neurolisteriosis cases in patients aged one month to under 50 years, five out of six (83%) occurred in immunocompetent individuals. Nosocomial-onset cases accounted for 31 (17%) of the non-perinatal listeriosis cases, including four cases that presented with febrile neutropenia. Ninety-five L. monocytogenes isolates from 16 hospitals were collected, and cgMLST analysis revealed multiple previously unnoticed disease clusters. In MLST analysis, clonal complex (CC) 1 was the most predominant, followed by CC3, CC87, CC155, and CC2. International transmission events in the late 20th century contributed to the emergence of CC87 isolates in Eastern Asian countries.
This study highlighted concerns regarding neurolisteriosis occurring in immunocompetent children and young adults, as well as the significance of non-clustered nosocomial onset listeriosis. Genomic analysis using whole-genome sequencing revealed previously unnoticed disease clusters and the unique population structure of L. monocytogenes in Japan.
我们开展了一项多中心回顾性研究，以描述日本境内李斯特菌病的临床特征以及致病性单核细胞增生李斯特菌分离株的基因组特征。
在这项回顾性观察队列研究中，我们纳入了2011年1月1日至2021年12月31日期间所有经微生物学确诊的李斯特菌病病例。我们收集了致病分离株并进行全基因组测序。使用多位点序列分型（MLST）、核心基因组MLST和基于单核苷酸多态性（SNP）的系统发育分析对分离株进行比较。
共纳入了来自22家医院的195例李斯特菌病病例，包括134例菌血症、43例神经型李斯特菌病、11例围产期感染和7例其他感染。在1个月至未满50岁患者的神经型李斯特菌病病例中，六分之五（83%）发生在免疫功能正常的个体中。医院感染病例占非围产期李斯特菌病病例的31例（17%），其中包括4例伴有发热性中性粒细胞减少症的病例。收集了来自16家医院共95株单核细胞增生李斯特菌分离株，核心基因组多位点序列分型（cgMLST）分析揭示了多个先前未被注意到的疾病聚集情况。在MLST分析中，克隆复合体（CC）1最为常见，其次是CC3、CC87、CC155和CC2。20世纪后期的国际传播事件导致了CC87分离株在东亚国家出现。
本研究强调了免疫功能正常的儿童和年轻人发生神经型李斯特菌病的问题，以及非聚集性医院感染性李斯特菌病的重要性。使用全基因组测序进行的基因组分析揭示了先前未被注意到的疾病聚集情况以及日本境内单核细胞增生李斯特菌独特的种群结构。
